宫颈尖锐湿疣中hTERC基因扩增和CD105表达的意义  

The expression and significance of h TERC and CD105 in cervical candyloma acuminatum

作　　者：冯雨苗[1] 孙澜[1] 刘标[2] 杨如[3] 孙文洁[4] 华纲[1] 陈朝潮[1] 赵斌斌[3] 施辛[5] 

机构地区：[1]南京医科大学附属苏州医院皮肤科,江苏苏州215001 [2]南京医科大学附属苏州医院病理科,江苏苏州215001 [3]南京医科大学附属苏州医院妇产科,江苏苏州215001 [4]宜兴市人民医院皮肤科,江苏宜兴214200 [5]苏州大学附属第二医院皮肤科,江苏苏州215004

出　　处：《南京医科大学学报（自然科学版）》2016年第8期979-982,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学校基金(2012NJMU162)

摘　　要：目的:探讨h TERC基因扩增和CD105在宫颈尖锐湿疣(candyloma acuminatum,CA)中的表达及其临床意义。方法:分别采用免疫荧光原位杂交(FISH)和免疫组化方法检测宫颈CA组织中h TERC基因扩增和CD105的表达。67例宫颈CA中单纯性CA 31例,CA-CINⅠ级19例,CA-CINⅡ级11例,CA-CINⅢ级6例;宫颈癌35例;正常宫颈黏膜24例作为对照。结果:宫颈正常黏膜、宫颈CA和宫颈癌中h TERC基因扩增率分别为0.0%、25.4%和80.0%,差异有统计学意义(P<0.01);CD105标记的微血管密度(MVD)亦存在明显差异(P<0.01)。宫颈CA中,h TERC基因扩增率和MVD呈显著正相关(P<0.05),均随CIN级别的升高而增加,其中CA-CINⅠ级与CA-CINⅡ级之间差异有统计学意义(P<0.01);两者表达在复发组均显著高于未复发组(P<0.05)。结论:h TERC基因扩增和CD105表达在宫颈CA复发并向高级别CIN转化过程中扮演着重要角色,对宫颈CA患者检测h TERC基因扩增和CD105表达有助于临床筛查高危CA患者。Objective：To evaluate the significance of h TERC gene amplification and CD105 expression in cervical condyloma acuminateum（CA）. Methods：Fluorescence in situ hybridization（FISH）and imunohistochemical staining were used to detect the expression of h TERC gene and CD105 in 67 cases of cervical CA,including 31 cases without CIN,19 CA-CIN grade Ⅰ,11 CA-CIN grade Ⅱ,and 6 CA-CIN grade Ⅲ; 35 cases of cervical cancer and 24 normal cervical mucosa were performed as controls. Results：The rates of h TERC gene amplification were 0%,25.4% and 80% in normal cervical mucosa,cervical CA and cervical cancer,respectively（P〈0.01）. Microvessel density（MVD） marked by CD105 antibody also had significant difference（P〈0.01）. In cervical CA,h TERC gene amplification and MVD showed a positive correlation（P〈0.05）and increased with increasing CIN grade. There were significant differences between CA-CIN Ⅰ and CA-CIN Ⅱ（P〈0.01）,as well as between the recurrence group and the non recurrence group（P〈0.05）. Conclusion：The expression of h TERC gene and CD105 may play an important role in the recurrence of cervical CA,and in the process of conversion to the high grade CIN. The detection of h TERC gene and CD105 is helpful in the clinical screening of high-risk patients with cervical CA.

关 键 词：宫颈尖锐湿疣 宫颈上皮内瘤变 H TERC基因 CD105 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]