去泛素化酶nonstop调控果蝇眼睛锥细胞发育的研究  

Deubiquitinating Enzyme nonstop Regulates Cone Cells Development in Drosophila Eye

作　　者：石伟杰[1] 沈成豪 林益[1] 谌玉婷 叶晓蕾[1] 冯颖[1] 林鑫华[1] Shi Weijie Shen Chenghao Lin Yi Chen Yuting Ye Xiaolei Feng Ying Lin Xinhua(School of Optometry, Wenzhou Medical University, Wenzhou 32502 7, Chin)

机构地区：[1]温州医科大学眼视光学院,温州325027

出　　处：《中国细胞生物学学报》2016年第8期907-917,共11页Chinese Journal of Cell Biology

基　　金：温州医科大学人才启动项目(批准号:QTJ08012);温州医科大学附属眼视光医院人才启动项目(批准号:KYQD141103)资助的课题~~

摘　　要：去泛素化酶是一类参与蛋白质稳定性调控的酶,广泛存在于真核生物细胞中,主要功能是水解底物蛋白质上的泛素链。去泛素化酶参与多种重要的生命活动,包括细胞周期调控、细胞信号转导、蛋白质降解、DNA修复及基因表达等,其异常表达与多种疾病的发生密切相关。该文以果蝇为研究对象,探讨去泛素化酶nonstop(not)对果蝇眼睛锥细胞发育分化的调控作用。结果表明,在not功能缺失的果蝇三龄幼虫眼睛成虫盘中,Cut蛋白质水平发生了显著下调;同时,在果蝇蛹期眼睛的分析结果显示,not功能缺失可导致锥细胞和初级色素细胞的分化异常。进一步研究发现,not功能缺失还可导致R1/R6光感受器细胞分化异常及转录因子D-Pax2表达水平异常。然而,not功能缺失并不影响转录因子Lozenge(Lz)的蛋白质水平及EGFR(epidermal growth factor receptor)和Notch信号通路的活性。综上所述,该文结果表明,not可能通过调控D-Pax2的表达水平与R1/R6光感受器细胞的分化影响锥细胞和初级色素细胞的分化。Deubiquitinating enzymes （DUBs） are kinds of protein involved in the regulation of protein stability in eukaryotic cells, and play important roles in cell processes including the regulation of cell cycle, cell signal transduction, protein degradation, DNA repair and gene expression. The function of DUBs is to cleave ubiquitin （Ub） from Ub-conjugated protein substrates, and dysfunction of DUBs causes many diseases. In the current study, we used Drosophila as a model to explore the function of deubiquitinating enzyme nonstop （not） in cone cells development. Our results showed that the protein level of Cut was reduced in not mutant cells in eye imaginal disc. In the pupal eye, knockout not affected the differentiation of cone cells and primary pigment cells. The further study showed that the expression level of D-Pax2 was changed and the differentiation of R1/R6 photoreceptor was abnormal in not mutant cells. However, the protein level of Lozenge （Lz） and the avtivity of epidermal growth factor receptor （EGFR） and Notch signaling were not affected in not mutant cells.Taken together, our result indicated that not may affect the differentiation of cone cells and primary pigment cells by regulating the expression level of D-Pax2 and the differentiation of R1/R6 photoreceptor.

关 键 词：去泛素化酶 NONSTOP 锥细胞 果蝇眼睛发育 

分 类 号：Q78[生物学—分子生物学] R339.14[医药卫生—人体生理学]