一株缬草根际稀有放线菌的分离鉴定及其天然产物生物合成基因的筛查  

Identification of an Rare Actinomycetes and Screening of Natural Products Biosynthesis Genes from Valerian Rhizosphere Soil

作　　者：安晓英[1] 马怡茗[1] 刘香香[1] 周童娜 颜华[1] 贾良辉[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《西北农业学报》2016年第7期1080-1086,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：中央高校基本科研业务费(Z109021426;Z109021432);上海交通大学微生物代谢国家重点实验室开放基金(MMLKF14-09);高校博士点基金(20120204120034;20120204120042)~~

摘　　要：采用梯度稀释涂布平板法分离缬草根际土壤中的放线菌,从高氏一号培养基上分离得到1株放线菌BJA-103,对该菌形态特征、培养特征、生理生化特征及16SrDNA基因序列进行鉴定,并用简并PCR方法对其多种天然产物生物合成关键酶基因进行筛查。经分类鉴定,初步确定BJA-103为Amycolatopsis coloradensis的一个菌株;PCR筛查结果显示,该菌株含有非核糖体肽合酶(NRPS)、Ⅰ型聚酮合酶(PKS-Ⅰ)、Ⅱ型聚酮合酶(PKS-Ⅱ)以及糖肽类抗生素合成关键酶P450单加氧酶的基因。Actinomycetes were isolated from valerian rhizosphere soil using dilution-plate method, one of them was identified based on morphological, cultural, physiological, biochemical characteristics and 16S rDNA gene sequence, and the key enzyme genes of natural products biosynthesis pathways were screened by degenerate PCR. The results showed that actinomycetes BJA-103 isolated from Gause Ⅰ medium was preliminarily identificated as a member of the genus Amycolatopsis coloradensis. PCR check-screening of its natural products biosynthesis genes revealed that it possessed the genes of non- ribosome polypeptide synthetase （NRPS）, polyketides synthetase Ⅰ （PKS- Ⅰ ）, polyketides syn- thetase Ⅱ （PKS- Ⅱ ） and glycopeptide P450 monooxygenase.

关 键 词：稀有放线菌 分类鉴定 16S RDNA 抗生素生物合成基因 

分 类 号：Q939.132[生物学—微生物学]