阳和化岩汤对乳腺癌模型大鼠Fas、FasL和Bcl-2表达的影响  

Effect of Yanghe Huayan Decoction on the Expressions of Fas/FasL and Bcl-2 in Breast Cancer Model Rats

作　　者：李静蔚[1] 陈翰翰[1] 时光喜[1] 杨丽爽 赵娜[3] 陈宏志[3] 刘晓菲[1] 

机构地区：[1]山东中医药大学附属医院,山东济南250014 [2]北京中医药大学枣庄医院,山东枣庄277000 [3]山东中医药大学,山东济南250355

出　　处：《山东中医杂志》2016年第9期819-823,共5页Shandong Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81573989;81403408);山东省自然科学基金(编号:ZR2011HL065)

摘　　要：目的:观察阳和化岩汤对乳腺癌大鼠Fas、FasL和Bcl-2表达的影响。方法:应用7,12-二甲基苯并蒽(DMBA)诱导大鼠建立乳腺癌模型,药物干预4周后,HE染色观察大鼠乳腺组织形态学变化,免疫组化法检测Fas、FasL、Bcl-2蛋白表达的阳性率,Q-PCR法检测Fas、Bcl-2 mRNA表达。结果:阳和化岩汤可明显降低大鼠乳腺癌的发生率(P<0.05),同时可升高Fas的表达(P<0.05)、降低FasL和Bcl-2的表达(P<0.05)。结论:阳和化岩汤具有预防乳腺癌的作用,其作用机制可能与调控细胞凋亡相关蛋白Fas、FasL和Bcl-2表达相关。Objective：To observe the effect of Yanghe Huayan decoction on the expression of Fas/FasL and Bcl-2 in breast cancer model rats. Methods：Breast cancer rat models were established by using 7,12-dimethylbenzanthracene（DMBA）,and then the rats were treated for 4 weeks. The rat breast tissue was observed after HE staining for morphological changes,and the positive rate of Fas/FasL and Bcl-2 expression were detected with immunohistochemistry,and expressions of Fasand Bcl-2 m RNA were detected with quantitative PCR. Results：Yanghe Huayan decoction could reduce the incidence rate of breast cancer in rats（P〈0.05）,increase the expression of Fassignificantly（P〈0.05） and decrease expressions of FasL and Bcl-2 significantly（P〈0.05）. Conclusion：Yanghe Huayan decoction could prevent breast cancer,and the mechanism might be involving regulating apoptotic related proteins Fas/FasL and Bcl-2.

关 键 词：阳和化岩汤 乳腺癌 细胞凋亡 FAS FASL BCL-2 

分 类 号：R285.5[医药卫生—中药学]