基于microRNA调控的HBeAg(-)轻度慢性乙型肝炎脾胃湿热证发生的分子机制研究  

Molecular Mechanism of Spleen- Stomach Damp- Heat Syndrome of Mild Chronic Hepatitis B with HBeAg Negative Based on microRNAs

作　　者：刘业方 张传涛[1,3] 黄群[1] 辜海英[3] 马喜桃[3] 黄晓群[1] 胡蓉[4] 

机构地区：[1]成都中医药大学,四川成都610075 [2]郫县中医医院,四川成都611730 [3]成都中医药大学附属医院,四川成都610072 [4]成都市传染病医院感染科,四川成都610066

出　　处：《辽宁中医杂志》2016年第9期1803-1807,I0001,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81202624,81102720)

摘　　要：目的:从microRNAs角度揭示HBe Ag(-)轻度慢乙型脾胃湿热证发生的分子机制。方法:将符合标准的病例分为HBe Ag(-)轻度慢乙肝脾胃湿热证组与HBe Ag(-)轻度慢乙肝无症状组,借助Agilent Human miRNA 8×60k微阵列芯片检测血浆中microRNAs表达谱,求得两组间的差异表达microRNAs谱(P<0.05),借助miRNA生物信息学分析软件预测其靶基因并对靶基因进行GO功能富集分析和pathway分析。结果:两组间的差异表达microRNAs共86条(P<0.05),37条上调,49条下调;GO分级及Pathway分析得到其功能主要涉及细胞黏附、生物合成过程的正/负调控、蛋白质定位、Wnt信号通路、RNA的代谢过程正/负调控基因表达的正/负调控、蛋白氨基酸的磷酸化、Notch信号传导途径、细胞凋亡、Hedgehog信号通路、T细胞受体信号通路等。结论:HBe Ag(-)轻度慢乙肝脾胃湿热证受特异性差异microRNAs调控,并涉及多个生命过程,其中Wnt信号通路可能是HBe Ag(-)轻度慢乙肝脾胃湿热证发生的关键分子机制。Objective：From microRNAs to discuss the expression and molecular mechanisms between spleen - stomach damp -heat syndrome and compliant asymptomatic group of mild chronic hepatitis B with positive HBsAg, HBeAg and HBcAb. Methods ：The patients were divided into spleen -stomach damp -heat syndrome group and compliant asymptomatic group of mild chronic hepatitis B with positive HBsAg, HBeAg and HBcAb. The fasting serum samples were collected and the expression profile of microRNAs in plasma was detected using Agilent Human miRNA 8 - 60 k microarrays. The data was analyzed using SPSS19. 0 software and online SAS system to obtain the differences expressed microRNAs spectrum between the groups （P 〈 0. 05）. Through the means of bioinformatics analysis software, analyze and predict the target genes with GO enrichment target genes KEGG pathway analysis. Results：The difference between the two types was totally 86 microRNAs expression （P 〈 0. 05 ）, 37 up- regulated,49 down- regulated. Analyze target genes GO enrichment and suggest regulating target genes and their participa- tion of cell adhesion, biosynthesis of positive/negative control, protein localization, Wnt receptor signaling pathways, RNA metabol- ic processes of positive/negative regulation of gene expression in the positive/negative control, protein amino acid phosphorylation and other vital processes. Pathway analysis results were mainly involved in Notch signaling pathway, apoptosis, Wnt signaling path- way, Hedgehog signaling pathway and T cell receptor signaling pathways. Conclusion： There are multiple differences of microRNAs between spleen - stomach damp - heat syndrome group and compliant asymptomatic group of mild chronic hepatitis B with positive HBsAg, HBeAg and HBcAb, which regulate more than one life processes and the mechanisms, importantly, Wnt signaling pathway may be a key molecular mechanism in spleen - stomach damp - heat syndrome group of mild chronic hepatitis B with positive HB- sAg, HBeAg and HBeAb.

关 键 词：HBeAg(-) 慢性乙型肝炎 脾胃湿热证 MICRORNAS 

分 类 号：R512.62[医药卫生—内科学]