牙周炎伴2型糖尿病患者非刺激性全唾液差异蛋白质组学研究  

A Proteomics of Differential Study on Non-stimulated Whole Salivas from Periodontitis Patients with Type 2 Diabetes Mellitus

作　　者：王侗飞 杨明[2] 郑燕华[3] 王继峰[2] 史琳涛[4] 杨福全[2] 汤楚华[1] 

机构地区：[1]解放军306医院全军口腔疾病诊治中心,北京100101 [2]中国科学院生物物理研究所,北京100101 [3]解放军306医院特种医学实验中心,北京100101 [4]解放军306医院全军糖尿病诊治中心,北京100101

出　　处：《解放军医药杂志》2016年第9期12-16,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：全军医学科技十二五课题(CWS11J175)

摘　　要：目的筛选并确定牙周炎伴2型糖尿病患者唾液中的差异性蛋白,以提高对牙周炎与2型糖尿病相互关系的认识。方法选取2015年6月—2015年12月的健康对照者16例、慢性牙周炎患者16例、2型糖尿病牙周健康者4例和牙周炎伴2型糖尿病患者16例被纳入研究。采集所有研究对象的非刺激性全唾液,用基于串联质谱标签标记结合高效液相色谱串联质谱技术进行蛋白质组学分析,筛选出牙周炎伴2型糖尿病的差异蛋白。结果本研究在唾液中共鉴定出630个蛋白质,与健康对照组比较,75个蛋白质表现出显著差异,46个上调,29个下调。通过UnitProt KB数据库比对,共筛选出13个可能与牙周炎伴2型糖尿病相关的蛋白质,分别为膜联蛋白A1、分泌性白细胞蛋白酶抑制剂、唾液凝集素、溶菌酶C、丝氨酸蛋白酶抑制剂A1、丝氨酸蛋白酶抑制剂A3表达上调,结合珠蛋白、前纤维蛋白1、钙结合蛋白S100P、葡萄糖-6-磷酸异构酶、乳酸脱氢酶、醛羧转移酶、碳酸酐酶1表达下调。结论本研究筛选出牙周炎伴2型糖尿病患者唾液中的13个差异蛋白质,可能作为其唾液生物标记物的候选蛋白。Objective To screen and identify differential proteins in non-stimulated whole salivas of periodontitis patients with type 2 diabetes mellitus so as to improve understanding of relationship between periodontitis and type 2 dia-betes mellitus. Methods A total of 16 healthy controls （ control group） , 16 patients with chronic periodontitis （ group A） , 4 patients with type 2 diabetes mellitus without periodontitis （ group B） and 16 patients with periodontitis accompa-nies by type 2 diabetes mellitus （ group C） from June to December 2015 were recruited in this study. Non-stimulated whole salivas of all groups were collected. A proteomics analysis was performed using technology based on tandem mass tags （ TMT） markers combined with high performance liquid chromatography-MS/MS （ HPLC-MS/MS） , and differential-ly expressed proteins of periodontitis accompanies by type 2 diabetes mellitus were screened. Results In this study, 630 proteins were identified. Compared with those in control group, 75 proteins showed significant differences including up-regulated 46 and down-regulated 29. According to UnitProtKB database, 13 proteins possibly associated with periodontitis accompanied by type 2 diabetes mellitus were identified. Annexin A1, secretory leucocyte protease inhibitor, saliva ag-glutinator, lysozyme C, serine proteinase inhibitors A1 and serine proteinase inhibitors A3 were up-regulated, while hap-toglobin, profilin-1, Ca binding protein S100-P, glucose-6-phosphate isomerase, lactic dehydrogenase, transaldolase, carbonic anhydrase 1 were down-regulated. Conclusion In this study, 13 differentially expressed proteins are screened in patients with periodontitis accompanied by type 2 diabetes mellitus, which may be used as candidate proteins of salivar-y biomarkers.

关 键 词：牙周炎 糖尿病 2型 唾液 蛋白质组学 

分 类 号：R587.1[医药卫生—内分泌] R781.42[医药卫生—内科学]