电镜超薄切片批量染色的新方法  

A new method for batch staining of ultra-thin sections

作　　者：王贝贝[1] 尹伟[1] 刘双双[1] 尚卫娜[2] 谢礼 杨平[1] 郭建胜[1] 王丽[1] 洪健[4] WANG Bei- bei YIN Wei LIU Shuang- shuang SHANG Wei- na XIE li YANG Ping GUO Jian- sheng WANG Li HONG Jian(Core Facilities, Zhejiang University School of Medicine, Hangzhou Zhejiang 310058 Life Sciences Institute,Zhejiang University, Hangzhou Zhejiang 310058 Zhejiang Academy of Agricultural Sciences,Hangzhou Zhejiang 310021 Center of Analysis and Measurement, Zhejiang University,Hangzhou Zhejiang 310058,China)

机构地区：[1]浙江大学医学院公共技术平台,浙江杭州310058 [2]浙江大学生命科学研究院,浙江杭州310058 [3]浙江省农业科学研究院,浙江杭州310021 [4]浙江大学农生环测试中心,浙江杭州310058

出　　处：《电子显微学报》2016年第5期462-465,共4页Journal of Chinese Electron Microscopy Society

基　　金：浙江省公益技术应用研究(浙江省科技厅资助项目No.2015C37049);浙江大学实验技术研究项目(No.SYB201401;No.SYB201407);浙江省高教实验室工作项目(No.YB201403);浙江省教育厅科研项目资助(No.Y201534707)

摘　　要：超薄切片染色是透射电镜生物样本制备中的关键环节。本文介绍了一种透射电镜超薄切片染色的新方法,利用简单自制的染色液防污存储与批量染色装置,可对超薄切片载网进行批量化染色,不但极大提高染色效率,而且减少了染色过程中染色液、样品的污染,达到染色液的重复利用,同时减少重金属对操作者的伤害和对环境的污染。The staining of ultra-thin sections is the most critical step in TEM biological sample preparation. Here we reported a new method for ultra-thin sections staining which could batch stain ultra-thin sections based on a simple equipment for storing the dye solution and batch staining. This method improved the efficiency for ultra-thin section staining and reduced the pollution of samples.Furthermore,it also protected environment as well as the operators from heavy metal toxicity.

关 键 词：超薄切片 批量染色 

分 类 号：Q-336[生物学]