鳗源创伤弧菌的鉴定与血清型分析  

Identification and serotypes analysis of Vibrio vulnificus from Anguilla anguilla

作　　者：许斌福[1] 龚晖[1] 李素一[1] 方冬兰[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《渔业研究》2016年第5期351-356,共6页Journal of Fisheries Research

基　　金：福建省科技厅公益类科研院所专项(2014R1019-6);福建省科技厅农业引导重点项目(2014N0010);福建省农科院双百项目(sbmd1627)

摘　　要：本研究对分离自发病鳗鲡的疑似创伤弧菌进行鉴定及血清型分析,经生化鉴定,从发病鳗鲡中得到7株创伤弧菌;设计了创伤弧菌溶血素基因(vlly)特异性引物,并对分离菌进行了PCR检测,创伤弧菌均可扩增出溶血素基因352 bp的目标片段,而非创伤弧菌未扩增出相应的片段;制备了创伤弧菌抗O抗原血清,凝集反应显示所得到的鳗源创伤弧菌抗O抗原血清不能与人源创伤弧菌1.1758发生凝集反应,菌株FJ03-X2抗O抗原血清可与大部分的鳗源创伤弧菌发生凝集反应。以上研究结果表明,基于溶血素基因设计的PCR引物具有良好的特异性,可用于创伤弧菌检测,鳗源创伤弧菌血清型不同于其他来源的创伤弧菌,菌株FJ03-X2的O抗原具有良好的免疫原性,可作为创伤弧菌疫苗研发的候选菌株。Seven strains of bacteria were isolated from the diseased eel, and which were identified as Vibrio vulnificus through 21 kinds of biochemical indexes analysis. The specific primers amplifying hemolysin gene （vlly） of Vibrio vulnificus were designed to detect the isolated bacteria genome DNA, the PCR results indicated that the specific fragments of 352 bp DNA could be amplified from the isolated Vibrio vulnificus, but could not amplify any positive band from non - Vibrio vulnificus bacteria. The mouse serum anti the 0 antigen of Vibrio vulnificus were prepared, and the assay of agglutination reaction showed that the serum anti - 0 antigen of Vibrio vulnificus from eel could not agglutinate to the strain 1. 1758 of Vibrio vulnificus isolated from human. However, the serum anti 0 antigen of FJ03 - X2 could agglutinate to the most strains of Vibrio vulnificus from Anguilla anguilla. These results suggested that the PCR primers based on hemolysin gene could specifically detect the strains of Vibrio vulnificus, the serovar of Vibrio vulnificus from eel was different from other sources, and the strain of FJ03 - X2 had good immunogenicity 0 antigen and could be as candidate strain for vaccine development of Vibrio vulnificus.

关 键 词：创伤弧菌 生物型 溶血素基因 血清型 

分 类 号：Q936[生物学—微生物学] S941.42[农业科学—水产养殖]