死亡谷芽孢杆菌NBIF-001防治灰霉病研究  

Studies on Controlling Effect of Bacillus vallismortis NBIF-001 on Gray Mold

作　　者：黄大野 周婷[2] 姚经武 刘晓艳 曹春霞 杨妮娜 胡洪涛 龙同 杨自文 

机构地区：[1]湖北省生物农药工程研究中心,湖北武汉430064 [2]武汉商贸职业学院,湖北武汉430205

出　　处：《中国蔬菜》2016年第10期63-66,共4页China Vegetables

基　　金：湖北省农科院竞争性项目(2015jzxjh04;2016jzxjh026);国家自然科学基金青年基金项目(31500428);湖北省农科院创新岗位项目(2016-620-000-001-038)

摘　　要：为明确死亡谷芽孢杆菌NBIF-001菌株对灰霉病的防治效果,采用平板对峙结合显微观察确定其对灰霉病菌的抑菌活性,并采用离体组织试验研究其对黄瓜叶部灰霉病及贮藏期番茄果实灰霉病的防治效果。结果表明,在平板对峙条件下NBIF-001对灰葡萄孢具有良好的抑菌活性,显微镜下观察到菌丝发生扭曲、肿胀和变形;离体试验结果表明NBIF-001对灰霉病具有良好的生防效果,对黄瓜叶片灰霉病和番茄果实灰霉病的防效分别达到85.91%和72.52%。In order to evaluate control effect of Bacillus vallismortis on gray mold of antagonistic bacterium strain NBIF-001,this paper determined its inhibition activity to the pathogen of Botrytis cinerea by dual culture method combined with optical microscope observation;and studied its controlling effects on cucumber leaf grey mold and tomato fruit grey mold by in vitro tissue test.The results showed that the strain NBIF-001 had high inhibition activity in dual culture.Optical microscope study revealed deformation of hyphal and enlargement of cytoplasmic vacuoles.In vitro tissue test shew that NBIF-001 exhibited better biological control effect on cucumber and tomato gray mold.The control effect reached 85.91% and 72.52%, respectively.

关 键 词：死亡谷芽孢杆菌 NBIF-001菌株 灰霉病 生防效果 

分 类 号：S476[农业科学—农业昆虫与害虫防治]