黄硬皮马勃子实体提取物抑菌活性  

Antimicrobial Activityof Scleroderma sinnamariense Fruit Body Extracts

作　　者：周会明[1,2] 张焱珍[1,2] 柴红梅[2] 张小雷[2] 刘璇 孔令舰 和娇娇 

机构地区：[1]滇西科技师范学院农学系,云南临沧677000 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650221

出　　处：《食用菌学报》2016年第2期65-69,共5页Acta Edulis Fungi

基　　金：国家食用菌产业技术体系(CARS-24);国家自然科学基金(31460014)资助

摘　　要：采用丙酮、正己烷、乙酸乙酯、正丁醇、石油醚、95%乙醇6种不同溶剂分别提取黄硬皮马勃(Scleroderma sinnamariense)子实体活性物质,并用纸片琼脂法测定黄硬皮马勃子实体提取物对4种细菌的抑菌活性。结果表明:提取物对大肠杆菌(Escherichia coli)的抑菌效果最好,其次是枯草芽孢杆菌(Bacillus subtilis),对金黄色葡萄球菌(Staphylococcus aureus)抑菌效果较弱,对四叠球菌(Micrococcus tetragenus)无明显抑菌效果;6种不同溶剂提取物对不同的细菌表现出相同抑菌规律,其中95%乙醇提取物的抑菌效果相对较强,抑菌圈直径可达30.83 mm,该溶剂的提取物对大肠杆菌、枯草芽孢杆菌的最小抑菌浓度分别为0.15、0.23mg/mL。Scleroderma sinnamariense fruit bodies were extracted by static immersion for 10 h at room temperature in 30 volumes of acetone,hexane,ethyl acetate,n-butanol,petroleum ether,95% ethanol or water,and the extracts were evaluated for antimicrobial activity against Escherichia coli,Bacillus subtilis,Staphylococcus aureus and Micrococcus tetragenus.Extracts prepared using organic solvents were relatively highly inhibitory of E coli and B.subtilis growth,especially those prepared with 95% ethanol,but none of the extracts had any effect on the growth of Micrococcus tetragenus.Minimal inhibitory concentrations of95% ethanol extracts were 0.15mg/mL and 0.23mg/mL for E.coli and B.subtilis,respectively.

关 键 词：黄硬皮马勃 提取物 抑菌作用 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]