LBL法评价桑黄菌株活性  

Bromothymol Blue Decolorization Rates and Evaluation of Mycelial Biomass Production,Flavone Levels and Antioxidant Activity of Ten Sanghuangporus Strains

作　　者：曲德辉 唐传红[1] 陈辉[1] 张赫男[1] 冯杰[1] 颜梦秋[1] 张劲松[1] 杨焱[1] 

机构地区：[1]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海201403 [2]上海海洋大学食品学院,上海201306

出　　处：《食用菌学报》2016年第2期84-87,109,119,共6页Acta Edulis Fungi

基　　金：国家十二五科技支撑课题(2012BAD36B05)资助

摘　　要：采用色度培养基(liquid medium supplemented with bromothymol blue and lactose,LBL)对10株桑黄菌株进行脱色能力评价及可见光谱分析,探究了菌株LBL法脱色率与液体发酵菌丝体生物量、菌丝体黄酮含量及菌丝体醇提物清除自由基活性的相关性。结果表明:脱色率较低的菌株,在液体传代发酵过程中生物量呈下降的趋势;脱色率与菌丝体生物量(第一代)、菌丝体黄酮含量、醇提物清除超氧阴离子IC_(50)值、醇提物清除过氧化氢IC_(50)值相关系数分别为0.44,0.73,-0.83和-0.55。数据说明可以通过LBL法快速评价桑黄菌株活性并筛选出衰退菌株。Bromothymol blue-decolorizing ability of metabolites produced during the growth of ten Sanghuangporus strains in a liquid culture medium（20% potato,2% NH4NO3,0.05% MgSO4·7H2O,0.15%KH2PO4,pH 7.0）supplemented with the 0.006% dye and 2% lactose（LBL medium）,together with mycelial biomass production,mycelial flavone levels,and free radical scavenging activity of ethanolic extracts of fungal mycelia,were determined.Mycelial biomass production decreased with increasing frequency of subculture for strains with low dye-decolorizing ability,and the correlation coefficients between decolorizing rate and mycelium biomass,mycelial flavone levels,and IC50 values for superoxide anion radical and H2O2 radical scavenging activity of ethanolic extracts of fungal mycelia,were 0.44,0.73,-0.83and-0.55,respectively.Our data revealed that dye-decolorization rates in LBL medium can be used to evaluate these biological features in strains of Sanghuangporus.

关 键 词：桑黄 菌株退化 溴麝香草酚蓝 抗氧化 

分 类 号：S567.39[农业科学—中草药栽培]