斑马鱼嗜水气单胞菌的鉴定和人工感染组织病理学研究  

Identification of Aeromonas hydrophila and Histopathological Observation of Artificial Infected Zebrafish

作　　者：林金杏 杨迟[1,2] 冯丽萍 胡建华 

机构地区：[1]上海实验动物研究中心,上海201203 [2]东华大学化学化工与生物工程学院,上海201620

出　　处：《生物技术通报》2016年第9期239-245,共7页Biotechnology Bulletin

基　　金：国家科技支撑计划课题(2013BAK11B02);上海市科技发展基金项目(15140901000)

摘　　要：从患病斑马鱼体内分离致病菌株并进行鉴定,观察人工感染分离菌株后组织病理学变化。通过对分离菌株ZF4形态特征、生理生化特性、保守基因和系统发育树建立等综合分析进行鉴定。结果显示,ZF4为β溶血的革兰氏阴性短杆菌,吲哚试验为阳性,发酵葡萄糖、蔗糖等糖类,AHA_0438、ASP、gyr B和16S r RNA等基因均为阳性,基于gyr B、16S r RNA基因系统发育分析结果显示ZF4为嗜水气单胞菌。人工感染后斑马鱼临床症状明显,发生肝脏组织变性、肠道内膜脱落、脾脏充血、胰腺间质纤维组织增生等组织病理学变化。斑马鱼体内分离的ZF4鉴定为致病性嗜水气单胞菌,且斑马鱼对该菌敏感,因此斑马鱼可作为研究嗜水气单胞菌的动物模型。The aims of this paper are to isolate and identify pathogenic strain from diseased zebrafish, and to observe the histopathological changes of artificial infected zebrafish. The isolated strain ZF4 was identified by comprehensive analysis of its morphology, physiological and biochemical characteristics, conserved gene and phylogenetic tree. The results showed that the ZF4 was gram negative bacillus, positive in indole test, fermented glucose, sucrose sugars, etc. The gene AHA_0438, ASP, gyrB and 16S rRNA of ZF4 were all positive. Based on gyrB and 16S rRNA gene sequence, clustering analysis result showed that ZF4 was Aeromonas hydrophila. The infected zebrafish presented obvious clinical symptoms, and histopathological changes were observed, such as the liver tissue degeneration, intestinal lining shedding, hyperemia of the spleen, pancreas fibrous tissue hyperplasia., etc. In conclusion, the ZF4 isolated from the diseased zebrafish was identified as pathogenicity A. hydrophila. Meanwhile, due to its susceptibility, zebrafish could be used as the animal model to study the A. hydrophila.

关 键 词：斑马鱼 嗜水气单胞菌 鉴定 人工感染 组织病理 

分 类 号：S941.4[农业科学—水产养殖]