枯草芽胞杆菌TR21防控粉杂1号香蕉枯萎病的效果和对根系抗性相关信号物质累积的影响  

Effects of Bacillus subtilis Strain TR21 Application on Fenza NO.1(Musa spp. ABB) Fusarium Wilt Morbidity and Resistance-related Signal Substances Accumulation in Roots

作　　者：张琳[1,2] 程萍[1,2,3] 喻国辉 王燕[2,3] ZHANG Lin CHENG Ping YU Guohui WANG Yan(College of Life Science, Zhongkai University of Agricultural and Engineering, Guangzhou 510225, China Zhuhai Modem Agriculture Development Center, Zhuhai 519075, China College of Life and Science Technology, Huazhong Agricultural University, Wuhan 430070, China)

机构地区：[1]仲恺农业工程学院生命科学学院,广州510225 [2]珠海市现代农业发展中心,珠海519075 [3]华中农业大学生命科学技术学院,武汉430070

出　　处：《中国生物防治学报》2016年第5期627-634,共8页Chinese Journal of Biological Control

基　　金：仲恺农业工程学院研究生创新资助项目(KA151495007);广东省农业科技推广项目(粤财农-2013-185号)

摘　　要：本文研究了枯草芽胞杆菌Bacillus subtilis TR21在大田对抗病品种粉杂1号枯萎病的防控效果及其与病原菌不同组合处理种苗根系抗性相关信号物质累积情况,评估了菌株TR21与粉杂1号结合减轻大田发病率的应用价值。田间试验设置清水对照区、菌株TR21可湿性粉剂喷叶区、菌株TR21可湿性粉剂喷叶+叶腋接种1次胶囊剂型区、菌株TR21可湿性粉剂喷叶+叶腋接种1次栓剂剂型区,并统计田间发病率、抽蕾率、断蕾率和产量。室内采用清水处理、菌株TR21叶腋接种、香蕉枯萎病菌FOC004孢子根系接种、菌株TR21叶腋接种后再在根系接种FOC004孢子的挑战接种4种处理方式,测定接种后不同时间点根系NO、H_2O_2和水杨酸(SA)变化。结果表明,TR21可湿性粉剂的不同处理均能降低粉杂1号发病率,其中喷叶区防效达72%,此外增加1次栓剂处理可以显著增加单株产量,并显著缩短粉杂1号生育期。对抗性信号物质测定发现,只有挑战处理能够在接种早期(4 h)激发NO的显著升高,并维持较长时间(72 h);3种处理均能够在早期(4 h)显著减少根系产生H_2O_2;病原菌和挑战处理在早期(4 h)能够激发根系SA含量显著升高。TR21叶腋喷施通过提高粉杂1号对枯萎病的抗性来降低田间发病率。Prevention and bio-control effects of Bacillus subtilis strain TR21 on banana fusarium wilt of resistant dwarf banana variety Fenza No.1（Musa spp. ABB）, and the accumulation of resistance-related signal substances in roots of the banana seedlings treated by TR21 were studied in this paper. Results showed, in field experiments, TR21 wettable powder application could reduce the fusarium wilt incidence of Fenza No.1 with control effect of 72%, and suppository added treatment could significantly shorten the growth period and increase yield per plant; In indoor experiment, challenge inoculation（FOC004 inoculation on roots after TR21 vaccinated on axil 24 h later） could inspire NO significantly increased in early vaccination（4 h）, and maintain 72 h; TR21 axil inoculation, FOC004 roots inoculation, and challenging inoculation could reduced producing H2O2 in roots in early 4 h; FOC004 roots inoculation, and challenging inoculation could inspire root SA contents significantly increased in early 4 h. TR21 axil inoculation reduced the incidence of banana fusarium wilt by improving the resistance ability of Fenza No.1 in field.

关 键 词：粉杂1号 香蕉枯萎病菌 枯草芽胞杆菌TR21 诱导抗性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]