兖州卷柏乙醇提取物对人食管癌裸鼠移植瘤细胞的抑制作用  

Effect of ethanol extract from selaginella involven on human carcinoma of esophagus cells transplanted in nude mice

作　　者：谢永华[1] 王冠明[1] 温扬敏[1] 杨维群 

机构地区：[1]泉州医学高等专科学校基础医学部,福建泉州362000 [2]泉州医学高等专科学校科研处,福建泉州362000

出　　处：《中国医药导报》2016年第27期27-30,42,共5页China Medical Herald

基　　金：福建省教育厅A类项目(JA13394)

摘　　要：目的探讨兖州卷柏乙醇提取物对人食管癌裸鼠移植瘤细胞的抑制作用。方法构建人食管癌裸鼠移植瘤模型,随机分成5组,每组各10只,分别灌胃给药等体积的兖州卷柏乙醇提取物高、中、低剂量组(400、200、100 mg/kg),并设置阳性对照(环磷酰胺,5 mg/kg)组和阴性对照(生理盐水,0.2 mL/只)组。每天给药1次。给药4 d后,开始测量并计算肿瘤体积。给药20 d后,处死裸鼠,称瘤重,并计算抑瘤率,肝脏、脾脏指数。结果实验结束后,兖州卷柏乙醇提取物高、中剂量组的肿瘤体积均比阴性对照组小且差异有统计学意义(P<0.01),但均比阳性对照组大且差异有统计学意义(P<0.05);低剂量组比阴性对照组小(P<0.05)。高剂量组食管癌肿瘤的重量比阴性对照组小(P<0.01),其抑瘤率为30.51%,但比阳性对照组大(P<0.05);中、低剂量均比阴性对照组小(P<0.05),抑瘤率分别为18.12%和9.25%。高剂量组肝脏指数比阴性对照组小(P<0.01);中剂量组肝脏指数也比阴性对照组小(P<0.05)。高剂量组脾脏指数比阴性对照组小(P<0.05)。结论兖州卷柏乙醇提取可以抑制人食管癌裸鼠移植瘤细胞的生长。Objective To investigate the inhibitory effect of ethanol extract from Selaginella Involven （ESI） on human carcinoma of esophagus cells transplanted in nude mice. Methods The models of nude mice were transplanted with human carcinoma of esophagus, and divided randomly into 5 groups with ten mice in each group. The groups of mice were treated by oral gavage daily as follow： high dosage group with ESI （400 mg/kg）, moderate dosage group with ESI （200 mg/kg）, low dosage group with ESI （i00 mg/kg）, positive control group with cyclophosphamide （5 mg/kg）; negative control group with normal saline （0.2 mL/mice）. The volumes of the tumor in the mice were＂ measured and calculated af- ter 4 days. After 20 days, all the mice were sacrificed for the measurements of the tumor weights and the tumor inhibi- tion rates. The liver and spleen indexes were measured. Results After the experiment, the tumor volumes in high dosage group and moderate dosage group were decreased compared with negative control group （P 〈 0.01）, which in- creased compared with positive control group （P 〈 0.05）; the tumor volumes in low dosage group were decreased com- pared with negative control group （P 〈 0.05）. The tumors in high dosage group were lighter than negative control group （P 〈 0.01） with the tumor inhibition rate of 30.51%, which was heavier than positive control group （P 〈 0.05）. The tu- mors in moderate and low dosage groups were lighter than negative control group （P 〈 0.05） with the tumor inhibition rates of 18.12% and 9.25% respectively. The liver indexes in high dosage group were lower than negative control group （P 〈 0.01）. The liver indexes in moderate dosage group were lower than negative control group （P 〈 0.05）. The spleen indexes in high dosage group were lower than negative control group （P 〈 0.05）. Conclusion The growth of transplanted tumor of human esophagus carcinoma in nude mice can be inhibited by ESI.

关 键 词：兖州卷柏乙醇提取物 食管癌 裸鼠 抑制作用 

分 类 号：R285[医药卫生—中药学]