结核分枝杆菌融合抗原38kD-Rv1419、16kD-Rv2041c在结核病血清学诊断中的应用价值  

The application value of fusionantigen 38kd-rv1419,16kd-rv2041c of mycobacterium tuberculosis in the serological diagnosis of tuberculosis

作　　者：郭洪娜[1] 

机构地区：[1]临沂市沂水中心医院,山东沂水276400

出　　处：《泰山医学院学报》2016年第7期738-741,共4页Journal of Taishan Medical College

摘　　要：目的探讨结核分枝杆菌融合抗原38k D-Rv1419、16k D-Rv2041c在结核病血清学中的诊断价值。方法以痰涂片、痰培养和标准试剂盒作为对照,应用化学发光法(chemiluminescence immunoassay,CLEIA)检测结核病组、非结核呼吸系病组、健康对照组血清标本中38k D-Rv1419、16k D-Rv2041c的抗体水平,评价其在结核病血清学中的诊断价值。结果 1肺结核病组38k D-Rv1419、16k D-Rv2041c的抗体水平(143621.0±23231.7,179731.1±16342.1)明显高于非结核呼吸疾病组(27417.5±2371.8,32066.2±3310.9)和健康对照组(35262.9±5764.2,22765.5±3695.7),(P<0.05)。238k D-Rv1419、16k D-Rv2041c检测抗结核抗体的敏感度(69.0%,72.0%)、高于痰培养(21.0%)、38 k D(20.0%)、16 k D试剂盒(26.0%),(P<0.05)。338k D-Rv1419、16k D-Rv2041c检测抗结核抗体的特异度(85.4%,83.8%)高于38k D(61.1%)、16k D试剂盒(50.0%),(P<0.05)。4联合应用38k DRv1419、16k D-Rv2041c融合抗原检测抗结核抗体的敏感度为89.0%,明显高于38k D-Rv1419(69.0%)、16k DRv2041c(72.0%),(P<0.05)。结论结核分枝杆菌融合抗原38k D-Rv1419、16k D-Rv2041c在结核病的血清学诊断上均具有较高的敏感度和特异度,其联合应用,能够提高抗结核抗体检测的敏感度。Objective： To study the application value of fusion antigen 38kD-Rv1419, 16kD- Rv2041 c of mycobacteri- urn tuberculosis in the serological diagnosis of TB. Methods ： With sputum smear and sputum culture and standard kit as a control, the IgG level of 38kD-Rv1419 and 16kD-Rv2041c in serum of TB group and non-TB respiratory disease group and health control group were detected by Chemiluminescence immunoassay, in order to study its value in serological diagnosis of TB. Results： （1） Antibody level of 38kD-Rv1419 and 16kD-Rv2041c in serum of TB group （143621.0± 23231.7, 179731.1 ± 16342. 1 ） were obviously higher than that in non-TB group （27417.5 ± 2371.8, 32066.2± 3310.9 ） and health control group （ 35262.9±5764. 2, 22765.5 ± 3695.7 ）, （ P 〈 0. 05 ） . （2） The sensitivity of 38kD-Rv1419 （69.0%）, 16kD- Rv2041c（72. 0% ） was higher than that of using sputum culture （21.0%）, 38kD kit （20.0%） and 16kD kit （26. 0% ） , （ P 〈 0. 05 ）. （3） The specificity of 38kD- Rv1419 （ 85.4% ）, and 16kD-Rv2041 c （ 83.8% ） was higher than that of 38kD kit （61.1% ） and 16kD kit （50. 0% ）, （P 〈 0. 05 ）. （4） The sensitivity of fusion antigen 38kD- Rv1419 combined with 16kD-Rv2041c was 89.0%, which was higher than that of 38kD-Rv1419 （69. 0% ） , and 16kD- Rv2041c （72. 0% ） , （P 〈 0. 05）. Conclusion： The sensitivity and the specificity of the fusion antigen 38kD-Rv1419 and 16kD-Rv2041c are high in serological diagnosis in TB, and the sensitivity of 38kD-Rv1419 plus 16kD-Rv2041c is in- creased in TB dignosis.

关 键 词：38k D-Rv1491 16k D-Rv2041c 化学发光酶免疫法 血清学诊断 

分 类 号：R52[医药卫生—内科学]