Wistar大鼠福氏佐剂性类风湿关节炎动物模型研究  被引量:8

Study on animal model of rheumatoid arthritis with frennd's complete adjuvant in Wistar rats

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作  者:朱玉芳 裴银辉 周芳媛 熊艳杰 董素宁 赵一帆 郑亚楠 

机构地区:[1]华北理工大学,河北唐山063000

出  处:《华北理工大学学报(医学版)》2016年第5期337-340,共4页Journal of North China University of Science and Technology:Health Sciences Edition

摘  要:①目的探讨Wistar大鼠佐剂性类风湿关节炎造模效果,为类风湿关节炎发生机制及治疗效果等相关研究中动物模型的应用提供实验依据。②方法将大鼠随机分为两组,分别为模型组和正常对照组,每组各20只。模型组于大鼠右足趾皮下注射福氏完全佐剂(CFA)0.15mL,造模后第7、14、21、28天对两组大鼠进行关节炎评分。测量每组大鼠右足趾关节肿胀度,分离脾脏并制备脾细胞悬液,计算脾细胞增殖率,ELISA法检测血清中IL-10与IL-17的含量。③结果模型组致炎后7天足趾肿胀达峰值,14天后再度肿胀,21天出现皮肤溃疡,28天后溃疡面进一步扩大。大鼠模型组第14天脾细胞增殖率高于第7天和第21天,差异有统计学意义(P<0.05),模型组不同时期脾细胞增殖率均高于对照组,差异有统计学意义(P<0.05);模型组第7、28天关节炎评分高于对照组,差异有统计学意义(P<0.05);第7、14、28天血清中IL-10与IL-17的含量高于对照组,差异有统计学意义(P<0.05)。④结论模型组关节肿胀明显,大鼠表现接近于人体的类风湿关节炎(RA),且造模过程简单、稳定,是研究RA的较理想模型。Objective To explore the effect of adjuvant rheumatoid arthritis model in Wistar rats, and provide experimental evidence for the application of animal models in the study of the mech- anism of rheumatoid arthritis and the evaluation of therapeutic effect. Methods The rats were ran- domly divided into two groups,model group and normal control group,20 in each group.Rats in mod- el group were injected with CFA 0.15mL. Then respectively to arthritis score of each group rats, measure the joint swelling of rats in each group,and calculate the spleen cell proliferation rate at the 7,14,21 and 28 days.Meanwhile heart blood and serum was separated to detect the content of serum IL-10 and IL-17 with ELISA method.Results Rats in model group induced inflammation after about 7 days the right toe swelling reached the peak.Then the right toe swelling agained about 14 days.The skin ulcer in rats occurred around 21 days.At 28 days,further pathological changed and ulcer surface to further expanded.In model group,The 14 days rats' spleen cell proliferation rate was higher than 7 days and 21 days,and the difference was significant ( P 〈0.05).The rats' spleen cell proliferation rate in model group was higher than that in significant ( P 〈0.05).The rats' arthritis sc the control group, and the difference was sig 17 at 7,14 and 28 days were higher than the control group at different times ore in model group at 7 days and nificant ( P 〈0.05).The content control group, the difference was ,and the difference was 28 days was higher than of serum IL-10 and IL- significant ( P %0.05).Conclusion The rats' joint swelling of model to human RA. Then the modeling process is rheumatoid arthritis. group is obvious, and the simple and stable, which performance of rats is an ideal animal is closer model of

关 键 词:类风湿关节炎 WISTAR大鼠 动物模型 福氏完全佐剂 

分 类 号:R392.9[医药卫生—免疫学]

 

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