丹参酚酸B通过Sirt-1对大鼠肝纤维化模型的影响  

Effect of liver fibrosis model in rats by salvianolic acid B resistant sirt-1

作　　者：吴航[1] 

机构地区：[1]菏泽医学专科学校药理学教研室,山东菏泽274000

出　　处：《华北理工大学学报（医学版）》2016年第5期349-352,共4页Journal of North China University of Science and Technology：Health Sciences Edition

摘　　要：①目的研究丹参酚酸B(SalB)抗大鼠肝纤维化分子的作用机制。②方法 48只大鼠随机分为4组:对照组(A组),DMN损伤组(B组),SalB空白给药组(C组),DMN损伤+SalB预处理组(D组)。B、D组大鼠腹腔注射DMN,每周3次,给药4周;D组大鼠在第一次注射DMN同时灌胃给予SalB溶液;C组也同时给该药,每天1次,连续给药6周。最后一次灌胃结束后禁食24小时,取肝脏标本,测定肝匀浆羟脯氨酸(HYP)水平。明胶酶谱法检测MMP-2、MMP-9活性,Western Blot检测AP-1和Sirt-1表达水平。③结果 B组大鼠肝脏HYP水平、MMP-2、MMP-9活性以及AP-1蛋白表达水平明显上升;与B组相比,D组大鼠肝脏HYP水平、MMP-2、MMP-9活性以及AP-1蛋白表达水平明显下降。另外,B组大鼠Sirt-1蛋白表达水平明显下降,而D组Sirt-1蛋白表达水平水平明显上升。④结论SalB具有明显的抗肝纤维化作用,其机制与SalB激活Sirt-1表达从而抑制MMP-2、MMP-9的活性密切相关。Objective To explore the molecule mechanism of anti-hepatic fibrosis by Salvianolic acid B（SalB）.Methods A total of 48 SD rats were randomly divided into four groups： （A） control group, （B） DMN group, （C） SalB group, （D） DMN＋SalB group.Rats in B and D group were intraper- itoneally injected with dimethylnitrosamine（DMN） three times a week,for four weeks. Rats in C and D group were lavaged by SalB once daily,for six weeks.The last time to fill the stomach after fasting for 24 hours,take the liver specimens.Then detected the level of HYP, MMP-2, MMP-9 ,AP-1 and Sirt-1. Results The levels of HYP,MMP-2,MMP-9 and AP-1 were significantly higher in the B group.How- ever compared with B group,the level of HYP, MMP-2, MMP-9, and AP-1 were significantly lower in D group.The level of Sirt-1 in D group was higher than B group.Conclusion SalB has obvious effect on anti liver fibrosis, the molecule mechanism is related to inhibit MMP-2 and MMP-9 by Sirt-1 activa- tion.

关 键 词：丹参酚酸B 肝纤维化 Sirt-1 MMP-2 MMP-9 

分 类 号：R657.3[医药卫生—外科学]