人脐带源间充质干细胞移植对再生障碍性贫血小鼠免疫保护的研究  

Immune protection effect of transplanting human umbilical cord-mesenchymal stem cells to aplastic anemia mice

作　　者：张英杰[1] 郝晓娜[1] 郝艳梅[1] 李玉云[1] 

机构地区：[1]蚌埠医学院医学检验系,安徽蚌埠233030

出　　处：《华北理工大学学报（医学版）》2016年第4期253-258,共6页Journal of North China University of Science and Technology：Health Sciences Edition

基　　金：安徽省教育厅自然科学重点项目(编号:KJ2012A198);安徽省教育厅自然科学一般项目(编号:KJ2015B050by)

摘　　要：①目的探讨人脐带源间充质干细胞(umbilical cord-mesenchymal stem cells,UC-MSCs)对免疫介导的再生障碍性贫血(AA)小鼠模型的治疗作用及其发挥免疫抑制作用的机制。②方法自然贴壁法分离、纯化UC-MSCs并进行体外培养和扩增;制备免疫介导的AA小鼠模型。模型制成后,将培养的UC-MSCs以1×106/kg的剂量经尾静脉注入模型鼠体内。RT-PCR法检测UC-MSCs对AA小鼠外周血及骨髓中单个核细胞γ-干扰素(IFN-γ)mRNA表达的影响,MTT法测定UCMSCs对异基因淋巴细胞增殖效应的影响,进一步探讨其发挥免疫抑制作用的机制。③结果输注UC-MSCs后AA小鼠外周血象、骨髓病理学特征等明显改善;UC-MSCs对异基因淋巴细胞增殖有明显抑制作用;与模型组比较,输注UC-MSCs可明显降低造血负调控因子IFN-γmRNA表达水平(P<0.05),恢复造血。④结论 AA小鼠输注UC-MSCs可使其骨髓造血衰竭情况明显改善;其机制与UC-MSCs可抑制T淋巴细胞的增殖及抑制造血负调控因子IFN-γmRNA表达有关。Objective To explore the treatment effect of transplanting human umbilical cord-mesenchymal stem cells（UC-MSCs）to aplastic anemia mice and mechanism of the immune protection effect.Methods The UC-MSCs derived from human umbilical cord were isolated,purified and culti-vated in vitro.Immune-mediated aplastic anemia models were established in mice.UC-MSCs were in-fused through the caudal vena.Allogeneic lymphocytes proliferation was detected by MTT assays.Ex-pression of IFN-γmRNA was detected by RT-PCR method to study the mechanism of treatment.Re-sults In UC-MSCs transplanting group,the quantity of WBC,RBC,Hb and Ret had been increasing than Model group.Compared with the control group,UC-MSCs transplanting could reduce the prolif-eration of lymphocyte,and could reduce the expression level of IFN-γmRNA.Conclusion UC-MSCs transplanting can reduce bone marrow failure of aplastic anemia mice by inhibiting the proliferation of lymphocyte and decreasing the expression level of IFN-γmRNA.

关 键 词：脐带源间充质干细胞 再生障碍性贫血 免疫保护 

分 类 号：R722.12[医药卫生—儿科]