PM_(2.5)对小鼠脑组织急性氧化损伤的影响研究  

Acute oxidative damage in mice brain after exposure to PM_(2.5)

作　　者：朱雨晴[1] 李金泉[1] 赵云[1] 宋静[1] 李潇潇[1] 蔡洁[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境生物医学实验室,湖北武汉430079

出　　处：《环境与健康杂志》2016年第8期679-682,F0003,共5页Journal of Environment and Health

基　　金：国家自然科学基金(51136002)

摘　　要：目的探讨PM_(2.5)对小鼠脑组织的急性毒性效应。方法将SPF级雄性Balb/c小鼠48只随机分为6组:对照组(生理盐水组)、生理盐水+褪黑素组、4μg/d PM_(2.5)组、20μg/d PM_(2.5)组、100μg/d PM_(2.5)组、100μg/d PM_(2.5)+褪黑素组,每组8只。各染毒组分别以0.1、0.5、2.5 mg/ml PM_(2.5)溶液进行气道滴注,每只小鼠每天滴注40μl,连续7 d;生理盐水+褪黑素组、100μg/d PM_(2.5)+褪黑素组每天以1.0 mg/ml褪黑素灌胃,灌胃剂量为10 mg/(kg·d),连续7 d。检测脑组织中活性氧自由基(ROS)、丙二醛(MDA)和还原型谷胱甘肽(GSH)的含量,并采用ELISA法检测脑组织中白细胞介素-4(IL-4)的水平。结果 100μg/d PM_(2.5)组小鼠脑组织ROS、MDA、IL-4含量高于对照组,GSH含量低于对照组,差异均有统计学意义(P<0.05或P<0.01);20μg/d PM_(2.5)组小鼠脑组织MDA含量高于对照组,差异有统计学意义(P<0.01)。100μg/d PM_(2.5)+褪黑素组小鼠脑组织ROS、MDA、IL-4含量低于100μg/d PM_(2.5)组,GSH含量高于100μg/d PM_(2.5)组,差异均有统计学意义(P<0.05或P<0.01)。结论 100μg/d PM_(2.5)气道滴注染毒可能对小鼠脑组织造成损伤,产生炎症反应,并通过氧化应激反应介导;褪黑素对PM_(2.5)引起的脑组织氧化损伤有一定的拮抗作用。Objective To evaluate the acute toxic effect in mice brain after seven days of exposure to PM_（2.5）. Methods Balb/c mice were randomly divided into six groups, eight in each： control group（saline group）, saline＋melatonin（MT） group,4 μg/d PM_（2.5）group, 20 μg/d PM_（2.5）group, 100 μg/d PM_（2.5）group and 100 μg/d PM_（2.5）＋MT group.PM_（2.5）exposure was conducted through intratracheal instillation and MT treatment was conducted through gavage, for seven consecutive days.Saline＋MT group and 100μg/d PM_（2.5）＋MT group were given 1.0 mg/ml MT and each mouse was received MT in a dose of 10 mg/（kg·d）. The contents of reactive oxygen species（ROS）, malonaldehyde（MDA） and glutathione（GSH） in mice brain were determined. The levels of interleukin-4（IL-4） in mice brain were also tested using ELISA. Results In the 100 μg/d PM_（2.5）group, the contents of ROS and MDA in mice brain significantly increased and the contents of GSH in mice brain were significantly decreased, the levels of IL-4 in mice brain were also increased compared with control group（P〈0.05,P〈0.01）. In the 20 μg/d PM_（2.5）group, the contents of MDA in mice brain significantly increased compared with control group（P〈0.01）. Compared with the 100 μg/d PM_（2.5）group,the 100 μg/d PM_（2.5）＋MT group showed a significant difference,the contents of ROS and MDA in mice brain significantly decreased and the GSH significantly increased,the levels of IL-4 were decreased（P〈0.05,P〈0.01）. Conclusion PM_（2.5）exposure at 100 μg/d may cause in flammation in mice brain and oxidative stress may be involved in it, and MT can play a protective role in the oxidative damage.

关 键 词：PM2.5 小鼠脑组织 氧化损伤 褪黑素 

分 类 号：R994.6[医药卫生—毒理学]