苏淮猪BMP4基因3'-UTR区克隆和多态性分析  

Cloning and polymorphism of BMP4 gene 3'-UTR in Suhuai pig

作　　者：马百全 魏天雨 吴艳 郭苹[3] 王钧顺 张建生[3] 邢军[3] 刘红林[1] 李齐发[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]淮阴种猪场,江苏淮安223322 [3]江苏农林职业技术学院,江苏句容212400

出　　处：《南京农业大学学报》2016年第5期819-824,共6页Journal of Nanjing Agricultural University

基　　金：江苏省农业科技自主创新资金(SCX(12)2097)

摘　　要：[目的]本试验旨在研究梅山猪和苏淮猪BMP4(bone morphogenetic protein 4)基因3'-UTR区序列特征,分析苏淮猪BMP4基因3'-UTR区SNP位点并了解苏淮猪和高繁殖力猪种梅山猪BMP4基因3'-UTR SNP位点多态性。[方法]采用克隆测序技术获得苏淮猪BMP4基因3'-UTR区序列;利用生物信息学方法分析其序列特征;利用池DNA测序技术筛选SNPs,采用AS-PCR技术分析苏淮猪和梅山猪SNPs位点多态性。[结果]苏淮猪BMP4基因3'-UTR区序列长度为267 bp,含有一个(AC)9微卫星位点和Poly A结构;在苏淮猪BMP4基因3'-UTR区20 nt处发现一个SNP,AS-PCR检测到3种基因型(GG、GC和CC);在苏淮猪中,GG型和G等位基因为优势基因型和优势等位基因,频率分别为0.815和0.887,而在高繁殖力猪种梅山猪中CC型和C等位基因为优势基因型和优势等位基因,频率分别为为0.667和0.770。[结论]获得了BMP4基因3'-UTR区序列,鉴定了一个新的SNP位点,发现BMP4基因3'-UTR区20G>C位点多态性可能与猪繁殖性能有关。[Objectives]The objective of this study is to identify the characterization of 3＇-UTR sequence of Suhuai pig bone morphogenetic protein 4（ BMP4） gene and the polymorphism of SNPs in 3＇-UTR of BMP4 between Suhuai pig and the high reproduction of Meishan pig. [Methods]The 3＇-UTR sequence of BMP4 was obtained by the cloning and sequencing and analyzed using bioinformatics methods. The SNPs of BMP4 were screened by sequencing of DNA pool and detected by the AS-PCR method between Suhuai pig and Meishan pig. [Results]The length of 3＇-UTR region of Suhuai pig BMP4 gene was 267 bp and contained a microsatellite loci of（ AC）9. Moreover,we found an SNP in the 20 nt in the 3＇-UTR of Suhuai pig BMP4 gene and detected three genotypes of GG,GC and CC by AS-PCR. The genotype of GG and G allele were higher in Suhuai pig and their frequency were 0.815 and 0.887,respectively. However,the genotype of CC and C allele were higher in high reproduction of Meishan pig and their frequency were 0.667 and0.770,respectively. [Conclusions]We isolate the 3＇-UTR sequence of Suhuai pig BMP4 gene and identify a new SNP in the 3＇-UTR of BMP4. The polymorphism of 20GC in the 3＇-UTR of BMP4 may be associated with reproductive performance.

关 键 词：苏淮猪 BMP4基因 3'-UTR SNP 多态性 

分 类 号：S828.8[农业科学—畜牧学]