沙蓬(Agriophyllum squarrosum)植株内固氮菌分布特征  

Distribution of Endogenous N-fixing Microbes in Agriophyllum squarrosum Plants

作　　者：李剑峰[1,2] 张淑卿[1,2] 苗阳阳[2] 黄廷州 张习会 

机构地区：[1]贵州师范学院喀斯特生境土壤与环境生物修复研究所,贵州贵阳550018 [2]甘肃农业大学草业生态系统教育部重点实验室,甘肃兰州730070

出　　处：《中国沙漠》2016年第4期1056-1062,共7页Journal of Desert Research

基　　金：国家自然科学基金项目(31300389;41561072);贵州省科学技术基金项目(黔科合J字[2014]2136)

摘　　要：沙蓬(Agriophyllum squarrosum)是适生于流动沙丘的先锋固沙植物,其植株氮积累量高。为探明固氮菌在沙蓬内的分布规律,对沙蓬植株各部位在不同生长时期内生固氮菌的数量分布进行了研究。结果表明:沙蓬植株内生固氮菌的数量分布在时间和空间上都具有很大的异质性。时间分布上,根内固氮菌的数量在生殖生长期明显高于其他时期;叶内固氮菌数量在营养生长期明显高于其他时期。在空间分布上,不同部位组织的内生固氮菌主要分布于宿主植株光合产物的源-库两端,除营养生长期固氮菌主要分布于叶内3.8×105 cfu·g-1(FW)外,均表现为根内固氮菌数量高于其他部位,并主要分布于须根;在植株地上部分,以营养生长期为典型代表,内生固氮菌更集中分布于其形态学的上端,如顶部的叶片和茎。Agriophyllum squarrosum is a moving sand-fixing plant which is adapted to growing in mobile dunes, and it has a high plant nitrogen accumulation. To investigate the distribution of nitrogen-fixing bac- teria in A. squarrosum whose different parts of different growth stages N-fixing microbes number of distri- bution. The results indicated that： A. squarrosum endogenous plant nitrogen-fixing bacteria have a great number of distribution heterogeneity in time and space. In time distribution, the number of nitrogen-fixing bacteria in the roots of reproductive growth was significantly higher than others; and the number of nitro- gen-fixing bacteria in the vegetative growth stage leaves was significantly higher than other periods. The spatial distribution of the number of different parts of the organization within N-fixing microbes main source in the plant photosynthetic products-both ends of the distribution of the library. In addition to the vegetative growth stage, nitrogen-fixing bacteria mainly distributed in leaves 3.8 X 10~ cfu. g I（FW） all showed signif- icantly higher than other parts of the root, and mainly existed in the fibrous; In the part of upper ground, making vegetative growth period as a typical representative, N-fixing microbes more concentrated on the upper end of its morphology, such as the top of the blade and stems.

关 键 词：沙蓬(Agriophyllum squarrosum) 内生固氮菌 时空分布 立枯体 

分 类 号：Q948.12[生物学—植物学] Q939.113