葛根素固体脂质纳米粒抗肝损伤作用研究  

Effects of Puerarin Solid Lipid Nanoparticles on Liver Injury

作　　者：刘娟[1] 郭宇[1] 胡孟洋 翟美芳 平洋[1] 张灵芝[1] 李雪欣[1] 于莲[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]山东大学临床医学院,济南250000

出　　处：《中国现代应用药学》2016年第9期1102-1106,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金项目(81274101)

摘　　要：目的制备葛根素固体脂质纳米粒(puerarin solid lipid nanoparticles,Pue-SLN),对Pue-SLN进行体外释药考察,并探讨Pue-SLN对CCl_4诱导的急性肝损伤大鼠的治疗作用。方法采用乳化-超声分散法制备Pue-SLN。大鼠腹腔注射CCl4造成急性肝损伤模型。48只大鼠随机分成6组:正常组、模型组、甘草酸二胺阳性对照组(13.5 mg·kg^(-1))、Pue-SLN高浓度组(27 mg·kg^(-1))、中浓度组(13.5 mg·kg-1)、低浓度组(6.75 mg·kg^(-1))。分别测定大鼠血清中丙氨酸转移酶(ALT)、碱性磷酸酶(ALP)、天门冬氨酸转移酶(AST)的活力,肝组织匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量,计算肝脏指数,并对肝组织进行组织形态学检查。结果 Pue-SLN各剂量组均能抑制肝损伤大鼠血清中ALT、AST、ALP活性,降低肝匀浆中MDA含量,增强SOD、GSH-PX活性,改善肝组织的病理形态。结论 Pue-SLN对CCl_4诱导的大鼠急性肝损伤具有治疗作用。OBJECTIVE To research the therapeutic effect of puerarin solid lipid nanoparticles（Pue-SLN） on liver injury in rats, prepare Pue-SLN and investigate drug release of Pue-SLN. METHODS Pue-SLN was prepared by emulsification ultrasonic dispersion method. Acute liver injury was induced by intraperitoneal injection of carbon tetrachloride in rats. Rats were randomly divided into 6 groups： normal control group, model group, glycyrrhizinate acid diamine positive control group（13.5 mg·kg^-1）, Pue-SLN groups with high concentration（27 mg·kg^-1）, middle concentration（13.5 mg·kg^-1） and low concentration（6.75 mg·kg^-1）. ALT, ALP, AST in serum and MDA, SOD, GSH-PX in liver tissue homogenate were detected. And then, liver index and obtained histomorphology examination of liver tissue were calculated. RESULTS Pue-SLN groups could significantly inhibit the activities of ALT, AST, ALP in serum, decrease the content of MDA in liver homogenate and increase the activities of SOD, GSH-PX in liver homogenate. Pue-SLN groups could improve the pathological of liver tissue via observing confirm the pathological section histomorphology of liver tissue. CONCLUSION Pue-SLN have therapeutic effects on acute liver injury induced by carbon tetrachloride in rats.

关 键 词：葛根素 固体脂质纳米粒 四氯化碳 急性肝损伤 

分 类 号：R965.2[医药卫生—药理学]