芘基探针1-(芘-1-基)-N,N-双(吡啶-2-基甲基)乙胺的合成及在焦磷酸作用下对Zn^(2+)的高效识别  

A Highly Selective and Sensitive Pyrene Derivative 1-(Pyren-1-yl)-N,N-bis(pyridin-2-ylmethyl)methanamine Chemosensor for Zinc(Ⅱ) in the Presence of Pyrophosphatic Acid

作　　者：沈薇[1] 王林[2] 陈敏[1] 吴旻[3] 包晓峰[4] 

机构地区：[1]南京农业大学理学院江苏省农药重点实验室,南京210095 [2]江苏科技大学环境与化工学院,镇江212003 [3]中国药科大学生科院,南京210009 [4]南京理工大学环境与生物工程学院,南京210094

出　　处：《有机化学》2016年第9期2204-2211,共8页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(No.21302098);中央高校基金(No.KJQN201415)资助项目~~

摘　　要：将二吡啶基乙基胺(DPA)与芘缩合还原后得到1-(芘-1-基)-N,N-双(吡啶-2-基甲基)乙胺(PYPA)可用作Zn^(2+)探针.在焦磷酸(PPi)存在下Me OH/H2O(3∶2,V/V,p H 7.20,HEPES 1.0 mmol/L)缓冲液中PYPA对Zn^(2+)显示出高度专一性,加Zn^(2+)后20 s在383 nm处荧光发射可达最大值,且检测不受各种阳离子或阴离子的干扰,其检测极限可低至30 nmol/L.活细胞成像研究表明PYPA可用于人肝细胞L-02中Zn^(2+)荧光标记.A pyrene derivative PYPA, namely, 1-（pyren-1-yl）-N,N-bis（pyridin-2-ylmethyl）methanamine （PYPA）, was synthesized to develop a chemosensor. The studies show that PYPA exhibits high sensitivity and selectivity toward Zn^2＋ over many other metal cations in the presence of pyrophosphatic acid （PPi）. It has a low detection limit of 0.030 nmol/L. Detection of Zn^2＋ was not interfered by various cations or anions. In the presence of PPi, over a 8-fold fluorescence enhancement at 383 nm was observed within 20 s. Cell studies further demonstrated that PYPA can be a potential probe to detect Zn^2＋ in human liver cells （L-02）.

关 键 词：芘衍生物 Zn^2+荧光探针 活细胞成像 

分 类 号：O657.3[理学—分析化学]