香蕉炭疽病拮抗菌Bs6602的诱变选育及防病作用研究  

Mutation breeding and control effect of banana anthracnose antagonistic strain Bs6602

作　　者：杜婵娟[1] 付岗[1] 潘连富[1] 叶云峰[2] 晏卫红[1] 

机构地区：[1]广西农业科学院微生物研究所,南宁530007 [2]广西农业科学院园艺研究所,南宁530007

出　　处：《植物保护》2016年第5期69-74,79,共7页Plant Protection

基　　金：广西自然科学基金项目(2013GXNSFBB053008);广西农业科学院基本科研业务费专项(2015YT79;2015JZ61)

摘　　要：为了提高拮抗菌对香蕉炭疽病的抑菌活性,本研究采用紫外线-亚硝酸钠复合诱变的方法对枯草芽胞杆菌Bs6602菌株进行诱变。结果表明,该菌株的最佳诱变条件为在距离20 W紫外灯50cm下照射15s,然后经300mmol/L NaNO2诱变处理60min。经过2次复合诱变,最终得到1株抑菌活性最强的6B8-5菌株,其抑菌活性比出发菌株提高83.80%。咪鲜胺对突变株6B8-5的最低抑菌浓度(MIC)为20.83μg/mL。连续转接7代,突变株6B8-5的抑菌活性无明显降低。6B8-5菌株的抑菌谱测试结果表明,其对13种植物病原真菌具有较强的抑菌活性,其中对多主棒孢霉的抑菌活性最强,抑菌圈直径达66.39mm。对香蕉炭疽病防治的试验结果表明,香蕉贮藏15d后,6B8-5菌株的防效明显高于出发菌株,为63.94%;6B8-5菌株与43 200倍咪鲜胺稀释液混用后,其防效最高达70.27%,与咪鲜胺1 350倍稀释液防效相当。In order to improve the antifungal activity of antagonistic bacteria on banana anthracnose,Bacillus subtilis strain Bs6602 was mutated by UV-NaNO2 compound.The results showed that the best mutation condition was irradiated by 20 W UV for 15 s when the irradiation distance was 50 cm,and then treated with 300 mmol/L NaNO2 for 60 min.After mutated twice,the strain 6B8-5 was screened for the strongest antifungal activity.Its antifungal activity was increased by 83.80% compared with the original strain.Minimal inhibitory concentration（MIC）of prochloraz against strain 6B8-5 was 20.83μg/mL.The antifungal activity of strain 6B8-5 was not reduced significantly after transferred for 7 generations continuously.The results of inhibition spectrum test showed that strain 6B8-5 had strong antifungal activity against 13 plant pathogenic fungi.Its antifungal activity against Corynespora cassiicola was the strongest,and the diameter of antifungal circle was 66.39 mm.The results of banana anthracnose control test showed that the control effect of strain6B8-5 was 63.94%,significantly higher than that of the original strain,after stored for 15 days.When the strain6B8-5 was mixed with the 43 200 times dilution of prochloraz,the control effect on banana anthracnose was 70.27%,similar to that with1 350 times dilution of prochloraz.

关 键 词：枯草芽胞杆菌 香蕉炭疽病 复合诱变 防治效果 

分 类 号：S436.681[农业科学—农业昆虫与害虫防治]