CRs、MMPs、VEGF在胰腺癌肿瘤相关成纤维细胞中的表达及其意义  

Expression of chemokine receptors,MMPs,and VEGF in pancreatic cancer fibroblast cell lines

作　　者：冯兰云[1] 陈联誉[1] 曲超[1] 王鹏[1] 陈顥 林钧华[1] 陈震[1] 孟志强[1] 刘鲁明[1] 

机构地区：[1]复旦大学附属肿瘤医院中西医结合科复旦大学上海医学院肿瘤学系,上海200232

出　　处：《中国癌症防治杂志》2016年第4期212-216,共5页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家自然科学基金青年基金资助项目(81102004);上海市卫生局中医药基金资助项目(2010QJ032A)

摘　　要：目的探讨趋化因子受体(chemokine receptors,CRs)、基质金属蛋白酶(matrix metalloproteinases,MMPs)、血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达与胰腺癌肿瘤相关成纤维细胞(cancer associated fibroblasts,CAFs)生物学行为的关系。方法用原代培养的方法建立胰腺癌CAFs和正常成纤维细胞(normal fibroblasts,NFs)。用Real-time PCR方法检测CRs(CXCR1、CXCR2、CXCR4、CXCR7)、MMPs(MMP1、MMP2、MMP7、MMP9)和VEGF m RNA的表达。结果成功建立胰腺癌CAFs和正常胰腺NFs。在CAFs和NFs中,趋化因子受体CXCR1、CXCR2、CXCR4、CXCR7 m RNA的表达均存在差异,CAFs表达量高于NFs。其中CXCR2、CXCR4 m RNA在两株细胞中的表达差异具有统计学意义(P=0.010,P=0.049)。MMPs和VEGF m RNA的表达同样存在差异,CAFs中MMP7和VEGF m RNA表达明显高于NFs,差异具有统计学意义(P=0.017,P=0.049);CAFs中MMP9 m RNA的表达明显低于NFs,差异具有统计学意义(P=0.012)。结论在胰腺癌CAFs中CRs、MMPs和VEGF m RNA的表达有差异,可能与其促侵袭转移能力存在一定的相关性。Objective To establish cancer associated fibroblast（CAF） lines and normal fibroblast （NF） lines and use them to investigate expression of chemokine receptors,MMPs and VEGF and thereby understand the biological behavior of CAFs in pancreatic cancer. Methods Primary cultures of CAFs and NFs were established,and mRNA levels of chemokine receptors,MMPs and VEGF were measured using RT-PCR. Results Expression of mRNAs encoding the chemokine receptors CXCR1,CXCR2,CXCR4, and CXCR7 was higher in CAFs than in NFs. This difference was statistically significant in the case of CXCR2 （P=0.010） and CXCR4 （P=0.049）. Similarly, MMP7 mRNA was expressed at significantly higher levels in CAFs （P=0.017）,as was VEGF（P=0.049）. Conversely,MMP9 mRNA levels were significantly higher in NFs （P=0.012）. Conclusion Differential expression of chemokine receptors, MMPs and VEGF in CAFs may help explain their aggressive behavior in pancreatic cancer.

关 键 词：胰腺肿瘤 肿瘤相关成纤维细胞 正常成纤维细胞 趋化因子受体 基质金属蛋白酶 血管内皮生长因子 

分 类 号：R735.9[医药卫生—肿瘤]