The sigma 54 genes rpoN1 and rpoN2 of Xanthomonas citri subsp. citri play different roles in virulence, nutrient utilization and cell motility  

作　　者：Gibson Kamau Gicharu SUN Dong-ling HU Xun FAN Xiao-jing ZHUO Tao WU Chuan-wan ZOU Hua-song 

机构地区：[1]Fujian Province Key Laboratory of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China [2]Huai'an Institute of Agricultural Sciences, Huai'an 223001, P.R. China

出　　处：《Journal of Integrative Agriculture》2016年第9期2032-2039,共8页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31171832);the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX(11)4056)

摘　　要：The sigma factor 54（σ^（54）） controls the expression of many genes in response to nutritional and environmental conditions. There are two σ^（54） genes, rpo N1（XAC1969） and rpo N2（XAC2972）, in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 were constructed in this study. All the mutants delayed canker development in low concentration inoculation in citrus plants. The bacterial growth of mutants was retarded in the medium supplemented with nitrogen and carbon resources. Under either condition, the influence degree caused by deletion of rpoN 2 was larger than the deletion of rpoN 1. Remarkably, the mutant ΔrpoN 1 showed a reduction in cell motility, while the mutant Δrpo N2 increased cell motility. Our data suggested that the rpoN 1 and rpoN 2 play diverse roles in X. citri subsp. citri.The sigma factor 54（σ^（54）） controls the expression of many genes in response to nutritional and environmental conditions. There are two σ^（54） genes, rpo N1（XAC1969） and rpo N2（XAC2972）, in Xanthomonas citri subsp. citri. To investigate their functions, the deletion mutants ΔrpoN1, ΔrpoN2 and ΔrpoN1N2 were constructed in this study. All the mutants delayed canker development in low concentration inoculation in citrus plants. The bacterial growth of mutants was retarded in the medium supplemented with nitrogen and carbon resources. Under either condition, the influence degree caused by deletion of rpoN 2 was larger than the deletion of rpoN 1. Remarkably, the mutant ΔrpoN 1 showed a reduction in cell motility, while the mutant Δrpo N2 increased cell motility. Our data suggested that the rpoN 1 and rpoN 2 play diverse roles in X. citri subsp. citri.

关 键 词：Xanthomonas citri subsp citri rpoN cell motility citrus canker full virulence 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]