SHBG基因在人滋养细胞凋亡中的作用  

Effect of SHBG gene on the apoptosis of human trophoblastic cells

作　　者：息小雪[1] 练思宇[1] 金镇[1] 孙磊[1] 孙倩[1] 冯冲[1] 王越[1] 张宝[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004

出　　处：《局解手术学杂志》2016年第10期711-715,共5页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金(81170591;81300511)

摘　　要：目的研究性激素结合球蛋白(SHBG)基因在人滋养细胞凋亡中的作用。方法用不同siRNA干扰沉默人滋养细胞SHBG基因的表达并分为6组,即正常对照组未经转染(Ⅰ组)、空白对照组仅转染脂质体(Ⅱ组)、阴性对照组转染Nonspecific siRNA(Ⅲ组)、转染组分别转染SHBGsiRNA-Ⅰ、SHBGsiRNA-Ⅱ、SHBGsiRNA-Ⅲ(Ⅳ、Ⅴ、Ⅵ组),然后通过Hoechst 33258染色检测人滋养细胞凋亡情况,Real-time PCR和Western blot测定SHBG mRNA及蛋白和凋亡相关抗体Caspase-3 mRNA及蛋白的表达水平。结果Ⅰ组、Ⅱ组、Ⅲ组SHBG和Caspase-3 mRNA及蛋白表达水平组间比较差异无统计学意义(P>0.05)。Ⅳ组、Ⅴ组、Ⅵ组SHBG和Caspase-3 mRNA及蛋白表达水平组间比较差异无统计学意义(P>0.05)。与Ⅰ组、Ⅱ组、Ⅲ组比较,Ⅳ组、Ⅴ组、Ⅵ组SHBG mRNA和蛋白表达量明显下调,Caspase-3 mRNA及蛋白表达水平升高,滋养层细胞凋亡增加,差异均具有统计学意义(P<0.05)。结论采用siRNA干扰技术能够降低人滋养细胞中SHBG基因表达量,导致人滋养细胞凋亡。Objective To investigate the effects of sex hormone-binding globulin （SHBG） gene in the apoptosis of human trophoblastic cells. Methods The siRNA specific-targeting SHBG gene was transfeeted into human trophoblastic cells and they were divided into six groups：trophoblasts without transfection in normal control groups（ group Ⅰ ） ;transfect liposome in blank control groups（ group Ⅱ ） ;transfect nonspecific siRNA in negative control groups （ group m ） ; transfect SHBG siRNA- Ⅰ , SHBG siRNA- Ⅱ , SHBG siRNA- m respectively in trans- fection group（ group ⅠⅢ Ⅳ Ⅴ ）. Hoechst 33258 dying method was used to detect cell apoptosis. SHBG and Caspase-3 mRNA profiling and the level of SHBG and caspase-3 protein were detected by real-time PCR and Western blot. Results There was no statistical significant difference in the gene expression and protein level of SHBG and caspase-3 in group Ⅰ , Ⅱ and Ⅰ （ P 〉 o. 05 ）. In IV, V and VI group, there was no statistical significant difference in the expression level of SHBG and caspase 3 （ P 〉 0.05 ）. Compared with group Ⅰ , Ⅱ and Ⅲ, the a- mount of SHBG gene expression decreased obviously, the caspase-3 mRNA and protein level increased obviously and the trophoblast cell ap- optosis increased markedly （P 〈 O. 05 ）. Conclusion Through siRNA interference technology can reduce SHBG gene expression in human trophoblastic cells, and it can lead to excessive apoptosis of human trophoblasts cells.

关 键 词：SIRNA干扰 人滋养细胞 SHBG基因 凋亡 

分 类 号：R711.7[医药卫生—妇产科学]