中药川芎对成骨细胞作用最佳浓度的体外实验  

Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro

作　　者：鲁巍[1] 郭睿[1] 赵刚[1] 

机构地区：[1]佳木斯大学口腔医学院,黑龙江省佳木斯市154002

出　　处：《中国组织工程研究》2016年第37期5483-5488,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:已有研究报道中药川芎具有促进成骨细胞增殖及分化的作用。目的:进一步观察中药川芎中川芎嗪成分对成骨细胞增殖及Ⅰ型胶原蛋白表达的作用。方法:以小鼠成骨细胞株MC3T3-E1为研究对象,在1,5,10,15,20 mg/L 5个不同质量浓度川芎嗪干预下分别应用CCK-8方法测定成骨细胞增殖能力,酶联免疫法测定碱性磷酸酶活性及Ⅰ型胶原蛋白表达。结果与结论:(1)1,5,10,15,20 mg/L川芎嗪对成骨细胞的增殖均有促进作用,与空白对照组比较差异有显著性意义(P<0.005);(2)1,5,10,15,20 mg/L川芎嗪可以提高成骨细胞内碱性磷酸酶活性,其中1,5,10 mg/L川芎嗪组与空白对照组比较差异有显著性意义(P<0.005),15,20 mg/L川芎嗪组与空白对照组比较差异无显著性意义(P>0.005);(3)1,5,10,15,20 mg/L川芎嗪均促进Ⅰ型胶原蛋白表达,与空白对照组比较差异有显著性意义(P<0.005);(4)结果表明,中药川芎提取物川芎嗪对成骨细胞增殖及Ⅰ型胶原蛋白表达均有促进作用,质量浓度为10 mg/L效果理想。BACKGROUND： It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation.OBJECTIVE： To further observe the effects of tetramethylpyrazine（Ligustrazine） extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I collagen expression. METHODS： Mouse osteoblast cell line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cell counting kit-8 assay, and alkaline phosphatase activity and type I collagen level measured using ELISA method. RESULTS AND CONCLUSION： Different concentrations of Ligustrazine all significantly promoted the osteoblast proliferation and type I collagen level compared with the blank control group（P〈0.005）. The activity of alkaline phosphatase in all Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group（P〈0.005）. These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I collagen expression, with the optimum concentration of 10 mg/L.

关 键 词：成骨细胞 细胞增殖 碱性磷酸酶 胶原I型 中草药 组织工程 组织构建 骨细胞 川芎嗪 I型胶原蛋白 

分 类 号：R318[医药卫生—生物医学工程]