不同孔隙率多孔钛铌合金的生物力学强度及细胞生物相容性  

Porous titanium-niobium alloys with different porosities: biomechanical strength and cytocompatibility

作　　者：王雷 李强 

机构地区：[1]陕西省中医院骨科,陕西省西安市710003

出　　处：《中国组织工程研究》2016年第38期5709-5715,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:钛铌合金具有较高的生物力学强度及较低的弹性模量,更加接近于人皮质骨,目前已被广泛作为牙科、骨科及人工髋关节植入材料。目的:观察不同孔隙率多孔钛铌合金的生物力学强度及细胞相容性。方法:利用粉末冶金技术制备40%、70%孔隙率的多孔钛铌合金,检测两种多孔钛铌合金的孔径、弹性模量及抗压强度。将兔骨髓间充质干细胞分别接种于40%、70%孔隙率的多孔钛铌合金表面,接种3,24,72 h,观察细胞黏附与增殖情况。结果与结论:(1)多孔钛铌合金孔径为200-500μm,两种均以350,400μm孔径为主,孔隙间三维连通,材料表面粗糙,70%孔隙率组孔隙呈蜂窝状,部分断裂,孔隙间连通;40%孔隙率组孔隙分布不均匀,孔隙间少有连通;两组钛铌合金均具有较高的抗压强度及较低的弹性模量,组间比较无差异;(2)两组钛铌合金均有利于细胞的黏附,70%孔隙率组表面的细胞黏附数量多于40%孔隙率组(P<0.05),且70%孔隙率组表面可见细胞外基质已形成,结果表明70%孔隙率多孔钛铌合金更有利于细胞的黏附。BACKGROUND： Titanium-niobium alloy holds high biomechanical strength and low elastic modulus that is close to the human cortical bone, so it has been extensively used as implant materials of dental, orthopedics and artificial hip joints. OBJECTIVE： To observe the biomechanical strength and cytocompatibility of porous titanium-niobium alloys with different porosities. METHODS： Porous titanium-niobium alloys with the porosity of 40% and 70% were prepared using powder metallurgy technology. Afterwards, the pore size, elasticity modulus and compressive strength of the two alloys were detected. Then the rabbit bone marrow mesenchymal stem cells were seeded onto the 40% and 70% porous titanium-niobium alloys, and cell adhesion and proliferation were observed at 3, 24 and 72 hours after inoculation, respectively. RESULTS AND CONCLUSION： Pore size of porous titanium-niobium alloys with rough surface was 200-500 μm, and both main pore size was 350 and 400 μm, and there was three-dimensional connectivity between the pores; in the 70% group, honeycombed pores were partly fractured, and connected mutually; pores distributed unevenly and there was little communication between the pores in the 40% group. In addition, both groups had high compressive strength and low elastic modulus, but there was no difference between the two groups. Furthermore, both two alloys promoted cell adhesion, and the number of cell adhesion in the 70% group was significantly higher than that in the 40% group（P〈 0.05）, and extracellular matrix could be found in the 70% group. These results show that porous titanium-niobium alloy with the porosity of 70% is significantly conducive to cell adhesion.

关 键 词：假体和植入物 弹性模量 组织工程 生物材料 材料相容性 多孔钛铌合金 人工关节置换 孔隙率 生物力学强度 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]