补阳还五汤对脑出血模型大鼠脑组织PI3K、Akt、Bcl-2、BAX蛋白表达的影响  

Effects of Buyanghuanwu decoction on the protein expression of PI3K, Akt, Bcl-2 and BAX in brain tissue of a rat model of cerebral hemorrhage

作　　者：吴晓光[1] 仇志富 孟杰[2] 祖炳学 李蒙蒙[4] 苗惠[4] 

机构地区：[1]河北省中医药抗痴呆重点研究室,承德医学院基础医学研究所,河北省承德市067000 [2]承德医学院附属医院,河北省承德市067000 [3]抚宁县第二人民医院,河北省秦皇岛市066300 [4]承德医学院,河北省承德市067000

出　　处：《中国组织工程研究》2016年第40期5933-5938,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省卫生厅资助项目资助(2014013);河北省高等学校重点学科病理学与病理生理学资助项目(2013);承德医学院重点资助项目(201607)~~

摘　　要：背景:补阳还五汤具有出色的神经保护作用,能够高效抑制脑缺血再灌注损伤导致的神经细胞凋亡。目的:研究补阳还五汤对脑出血大鼠血肿周围神经元凋亡的影响及作用机制。方法:将72只成年SD大鼠随机分为假手术组、模型组、补阳还五汤组、银杏叶片组,除假手术组,其余3组建立大鼠脑出血模型,造模后第2天开始,补阳还五汤组、银杏叶片组分别灌胃给予补阳还(kg五汤26 g/·d)、银杏叶片3.5 mg/(kg·d),假手术组及模型组灌胃给予等量生理盐水,连续给药14 d。末次给药后,取脑组织,TUNEL法检测神经元凋亡,免疫组织化学法检测PI3K、Akt、Bcl-2、BAX蛋白表达,干湿重法检测脑组织水含量,伊文思蓝法检测血脑屏障通透性。结果与结论:(1)与假手术组比较,模型组大鼠脑组织神经元凋亡数目、脑组织水含量、伊文思蓝含量及PI3K、Akt、Bcl-2、BAX蛋白表达增加(P<0.05);(2)与模型组比较,补阳还五汤组、银杏叶片组凋亡神经元数目、BAX蛋白表达、脑组织水含量、伊文思蓝含量显著降低(P<0.05),PI3K、Akt、Bcl-2蛋白表达明显升高(P<0.05);(3)结果表明:补阳还五汤可能通过降低血脑屏障通透性,减轻脑水肿,激活PI3K/Akt信号转导通路,进而调控Bcl-2与BAX蛋白表达比值,起到抑制脑出血神经元凋亡、保护脑组织的作用。BACKGROUND： Buyanghuanwu decoction has excellent neuroprotective effect and can efficiently suppress nerve cell apoptosis caused by cerebral ischemia-reperfusion injury. OBJECTIVE： To investigate the effect and mechanisms of Buyanghuanwu decoction on neuronal apoptosis around hematoma in cerebral hemorrhage rats. METHODS： Seventy-two adult Sprague-Dawley rats were randomly divided into sham operation group, model group, Buyanghuanwu decoction group, and Ginkgo biloba group. Except the sham operation group, rat models of cerebral hemorrhage were established in other three groups. At 2 days after modeling, rats in the Buyanghuanwu group and Ginkgo biloba group were given Buyanghuanwu decoction 26 g/（kg · d）and Ginkgo biloba 3.5 mg/（kg · d） daily by gavage, for 14 consecutive days. Rats in the sham operation group and model group received an equal volume of saline for 14 consecutive days. After the last administration, brain tissue was obtained. TUNEL assay was utilized to detect neuronal apoptosis. Immunohistochemistry was used to detect PI3 K, Akt, Bcl-2, and BAX protein expression. Wet and dry weight method was used to detect brain water content. Evans Blue assay was utilized to determine blood-brain barrier permeability. RESULTS AND CONCLUSION：（1） Compared with the sham operation group, the number of apoptotic neurons, brain water content, Evans blue content and PI3 K, Akt, Bcl-2, BAX protein expression increased in the model group（P〈0.05）.（2） Compared with the model group, the number of apoptotic neurons, BAX protein expression, brain water content and Evans blue content were significantly reduced in the Buyanghuanwu group and Ginkgo biloba group（P〈0.05）, but PI3 K, Akt and Bcl-2 protein expression was significantly increased（P〈0.05）.（3） Results suggested that Buyanghuanwu decoction inhibited neuronal apoptosis and protected brain tissue by reducing blood-brain barrier permeability, cerebral edema, and by activating PI3K/Akt signaling pathway, regulating Bcl-2 and

关 键 词：实验动物 神经损伤与修复动物模型 补阳还五汤 PI3K/AKT Bcl-2、BAX 组织神经细胞凋亡 脑水肿 血脑屏障 

分 类 号：R318[医药卫生—生物医学工程]