MRI-三维重建动态测量颅脑创伤模型大鼠的空腔变化  

Dynamic changes of brain cavity in rats after traumatic brain injury detected by MRI-based three-dimensional reconstruction

作　　者：符锋[1] 赵明亮[1] 李晓红[1] 陈翀[1] 王丽娜[1] 孙洪涛[1] 涂悦[1] 张赛[1] 

机构地区：[1]脑创伤与神经疾病研究所,武警后勤学院附属医院脑科医院,天津市神经创伤修复重点实验室,天津市300162

出　　处：《中国组织工程研究》2016年第40期5946-5952,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81301050,81401067,81471275,81271392);中国博士后基金项目(2013M542583)~~

摘　　要：背景:目前针对颅脑创伤后空腔的研究工作大多采用尸体标本或者针对实验动物取材后进行观察,无法实现动态评估空腔变化。目的:探讨三维重建在颅脑创伤预后评估中的应用效果。方法:取5只雄性SD大鼠,使用电子脑皮质挫伤撞击仪制备颅脑创伤模型,分别于造模后第1天、第1个月、第2个月和第3个月获取大鼠颅脑MRI扫描数据,应用Mimics16.0软件重建颅脑创伤后空腔三维模型,在Meshmixer软件中分析颅脑创伤后空腔变化情况。结果与结论:(1)重建模型图像轮廓清晰,均能从不同侧面和视角进行颅脑创伤空腔的观察和测量;(2)颅脑创伤后不同时间点的空腔体积及空腔表面积,除了第2个月与第3个月差异无显著性意义外(P>0.05),其他各个时间点之间两两比较差异均有显著性意义;(3)空腔变化结果提示,颅脑创伤后第3个月空腔形状逐渐规则;(4)基于MRI扫描数据,结合三维重建软件Mimics与模型分析软件Meshmixer,可方便、快捷地获取空腔模型,为动态评估颅脑创伤后空腔变化提供一种直观的方法。BACKGROUND： Currently, morphological observations of brain cavity after traumatic brain injury（TBI） via cadavers or animal specimen are difficult to obtain dynamic changes. OBJECTIVE： To explore the application effect of MRI-based three-dimensional（3D） reconstruction for evaluating the prognosis of TBI. METHODS： Five male Sprague-Dawley rats were enrolled to establish TBI models by Electronic Cortical Contusion Injury（eC CI）, and scanned by 3.0T MRI with Rat-coil to obtain the DICOM date of brain at 1 day, 1, 2 and 3 months after modeling. Brain cavities were 3-dimensionally reconstructed by Mimics16.0 software, and analyzed in the Meshmixer software. RESULTS AND CONCLUSION：（1） The outline of reconstruction model image was clear, and could be observed and measured from different sides and perspectives.（2） The cavity volume and surface area at different time points after TBI showed significant differences between each other except that at 2 and 3 months（P〈0.05）.（3） The results of cavity change suggested that the cavity tended to be regular after 3 months of TBI.（4） In conclusion, 3D reconstruction software Mimics combining with model analysis software Meshmixer can conveniently and quickly obtain the cavity model, and provide an intuitive way for evaluating the dynamic variations of the brain cavity after TBI.

关 键 词：实验动物 神经损伤与修复动物模型 三维重建 核磁共振 MIMICS 颅脑创伤 动态测量 空腔 Meshmixer DICOM 体积 表面积 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]