正交法筛选组穴对脑缺血再灌注损伤模型大鼠细胞外信号调节激酶信号转导通路的影响  

Effects of electroacupuncture at points selected by orthogonal experiment on the extracellular signal regulated kinase signal pathway in a rat model of cerebral ischenia-reperfusion injury

作　　者：冯毅慧[1] 朱志华[1] 吴春晓[2] 周国平[2] 

机构地区：[1]广西中医药大学附属瑞康医院针灸科,广西壮族自治区南宁市530011 [2]南方医科大学,广东省广州市510515

出　　处：《中国组织工程研究》2016年第40期5953-5958,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81173355)~~

摘　　要：背景:既往电针治疗脑缺血再灌注损伤观察的针刺穴位搭配较少。目的:探讨电针正交实验所选组穴对脑缺血再灌注损伤后细胞外信号调节激酶信号通路的影响。方法:150只SD大鼠随机等分为5组,假手术组只暴露大脑中动脉;模型组及正交1,2,3号组建立脑缺血再灌注模型。正交1,2,3号组采用正交法筛选的穴位组合进行电针刺激。正交1号组为百会、曲池、足三里、尺泽,正交2号组为百会、足三里、尺泽、三阴交,正交3号组为合谷、足三里、尺泽、三阴交。结果与结论:与模型组相比,正交1,2,3号组大鼠神经功能缺损评分降低,脑梗死面积缩小,脑缺血区微血管密度数量及缺血侧皮质和海马CA1区中磷酸化细胞外信号调节激酶表达水平增加,且正交3号组变化最为显著。提示电针经正交法筛选的合谷、足三里、尺泽、三阴交能有效减少脑缺血再灌注大鼠的梗死面积,且该效应可能与其调节受损脑组织中细胞外信号调节激酶信号通路密切相关。BACKGROUND： Acupuncture point matching is little reported in the electroacupuncture for cerebral ischemia-reperfusion injury. OBJECTIVE： To explore the influence of electroacupuncture acupoints selected by orthogonal experiment on rats with cerebral ischemia reperfusion and extracellular signal regulated kinase（ERK） signal pathway. METHODS： 150 Sprague-Dawley rats were randomly divided into five groups： sham operation group rats were exposed the middle cerebral artery only; models of cerebral ischemia reperfusion were established in the rats in model and orthogonal groups. Then rats in the orthogonal groups 1（Baihui（DU 20）, Quchi（LI 11）, Zusanli（ST 36）, and Chize（LU 5））, 2（Baihui（DU 20）, Zusanli（ST 36）, Chize（LU 5）, and Sanyinjiao（SP 6）） and 3（Hegu（LI 4）, Zusanli（ST 36）, Chize（LU 5） and Sanyinjiao（SP 6）） were given electroacupuncture at acupoints selected by orthogonal experiment, respectively. RESULTS AND CONCLUSION： Compared with the model group, in all orthogonal groups, the neurological function deficit scores were decreased, infarct volume was smaller, the number of microvessel density in the ischemia region and the p-ERK expression level in the cortex and hippocampal CA1 region of ischemic hemisphere were increased, and significant changes were found in the orthogonal group 3. These findings indicate that electroacupuncture at points of Hegu（LI 4）, Zusanli（ST 36）, Chize（LU 5） and Sanyinjiao（SP 6） selected by orthogonal experiment can significantly reduce the infarct volume of cerebral ischemia-reperfusion injury in rats possibly by regulating the ERK signaling pathway.

关 键 词：实验动物 神经损伤与修复动物模型 脑缺血再灌注 正交实验 细胞外信号调节激酶信号通路 微血管密度 细胞凋亡 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]