脊髓损伤模型大鼠胶质细胞反应性增生的变化规律及意义  

Reactive hyperplasia of glial cells induced by spinal cord injury in a rat model

作　　者：樊旭辉[1] 杨波[1] 胡祥 关方霞[3] 

机构地区：[1]郑州大学第一附属医院神经外科,河南省郑州市450052 [2]深圳市北科细胞工程研究所,广东省深圳市518000 [3]郑州大学生命科学学院,河南省郑州市450001

出　　处：《中国组织工程研究》2016年第40期6001-6006,共6页Chinese Journal of Tissue Engineering Research

基　　金：2011年国家自然科学基金项目(81171177);河南省高校科技创新团队支持计划(15IRTSTHN022);河南省科技创新杰出人才(154200510008)~~

摘　　要：背景:脊髓损伤后神经结构重建及修复成为关注焦点。稿目的:观察脊髓损伤后胶质细胞的变化规律。方法:取42只成年雄性SD大鼠,以随机数字方法分为7组,每组6只,正常对照组不进行任何干预;假手术组仅打开椎板,不损伤脊髓;脊髓损伤后1,7,14,21,28 d组,建立脊髓损伤模型,分别于脊髓损伤后相应时间点处死。采用BBB评分法评估各组大鼠后肢运动功能,取完整脊髓组织,进行苏木精-伊红染色、免疫组织化学染色与免疫荧光染色。结果与结论:(1)BBB评分:正常对照组与假手术组运动功能正常。脊髓损伤后1 d完全瘫痪,7 d后肢运动开始恢复,14 d明显恢复,21,28 d后肢运动功能与14 d无明显差异;(2)苏木精-伊红染色:脊髓损伤后1 d,髓内弥漫性出血,细胞大量坏死;伤后7 d,髓内出血逐渐吸收,炎症细胞浸润,部分细胞形成空泡;伤后14 d,出血完全吸收,脊髓结构破坏,囊腔形成;伤后28 d,脊髓结构完全破坏,巨大空洞形成,局部大量瘢痕组织形成;(3)免疫组织化学染色:脊髓损伤后,星形细胞增生、突触增加,14 d时最为明显;7 d可见轴突间隙稍增大,结构紊乱,21 d髓鞘结构破坏;(4)免疫荧光染色:脊髓损伤14 d,损伤局部出现大量胶质纤维酸性蛋白阳性/神经巢蛋白阳性细胞;(5)结果表明:脊髓损伤早期,损伤周围星形胶质细胞细胞突起和胞体肥大、增殖,胶质纤维酸性蛋白和神经巢蛋白表达上调,有利于脊髓损伤修复。BACKGROUND： The nervous reconstruction and repair after spinal cord injury have become a research hotspot. OBJECTIVE： To investigate the change rule of neurogliocyte reactive hyperplasia after spinal cord injury. METHODS： Forty-two adult male Sprague-Dawley rats were selected and equivalently randomized into seven groups： normal control group（no intervention）, sham operation group（lamina decompression） and operation groups（postoperative 1, 7, 14, 21 and 28 days）. After the establishment of spinal cord injury models, the rats were sacrificed at each corresponding time point. The functional recovery of the rat hind limbs was evaluated by Basso, Beattie and Bresnahan scores, and complete spinal cord tissue was removed to undergo hematoxylin-eosin staining, immunohistochemistry staining and immunofluorescence staining. RESULTS AND CONCLUSION：（1） Basso, Beattie and Bresnahan scores showed that rats in the normal control and sham operation groups had normal neurologic function. Rats at 1 day after spinal cord injury paralyzed completely, the neurologic function of hind limbs began to recover gradually at the 7th day, and the recovery became most obvious at the 14 th day, which had no significant differences compared with the 21 st and 28 th days.（2） Hematoxylin-eosin staining found that the diffuse hemorrhage and neuronal necrosis were observed in the injured area at 1 day after operation; inflammatory cell infiltration and some vacuoles appeared at the 7th day, and the hemorrhage was absorbed gradually; the hemorrhage disappeared completely and capsule cavity formed at the 14 th day; up to the 28 th day, spinal cord structure was completely destroyed and that was replaced by cicatricial tissue accompanying with a large cavity.（3） Immunohistochemistry staining showed that the astrocyte in damaged area proliferated with the cell synapse increasing, which was most overt at the14 th day; the axon clearance widened and the structure was in disorder at the 7th day, and the myelin sheath in

关 键 词：实验动物 神经损伤与修复动物模型 胶质细胞反应性增生 胶质纤维酸性蛋白 髓鞘碱性蛋白 免疫荧光染色 反应性星形胶质细胞 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]