Limited ecological risk of insect-resistance transgene flow from cultivated rice to its wild ancestor based on life-cycle fitness assessment  

作　　者：Lei Li Xiao Yang Lei Wang Huanxin Yan Jun Su Feng Wang Bao-Rong Lu 

机构地区：[1]Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, Institute of Biodiversity Science, Fudan University [2]Fujian Province Key Laboratory of Genetic Engineering for Agriculture, Fujian Academy of Agricultural Sciences

出　　处：《Science Bulletin》2016年第18期1440-1450,共11页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31330014);the National Program of Development of Transgenic New Species of China (2016ZX08011006)

摘　　要：Ecological impact caused by transgene flow from genetically engineered （GE） crops to their wild rela- tives is largely determined by the fitness effect brought by a transgene. To estimate such impact is critical for the eco- logical risk assessment prior to the commercialization of GE crops. We produced F1 and F2 hybrid descendants from crosses of two insect-resistant GE rice lines （Bt, Bt/CpT1） and their non-GE rice parent with a wild rice （Oryza ruff- pogon） population to estimate the transgenic fitness. Insect damages and life-cycle fitness of GE and non-GE crop- wild hybrid descendants as well as their wild parent were examined in a common-garden experiment. No significant differences in insect damages were observed between the wild rice parent and GE hybrid descendants under high- insect pressure. The wild parent showed significantly greater relative survival-regeneration ratios than its GE and non-GE hybrid descendants under both high- and low-in- sect pressure. However, more seeds were produced in GE hybrid descendants than their non-GE counterparts under high-insect pressure. Given that the introduction of Bt and Bt/CpT1 transgenes did not provide greater insect resistance to crop-wild hybrid descendants than their wild parent, we predict that transgene flow from GE insect-resistant rice to wild rice populations may not cause considerable ecolog- ical risks.Ecological impact caused by transgene flow from genetically engineered(GE) crops to their wild relatives is largely determined by the fitness effect brought by a transgene. To estimate such impact is critical for the ecological risk assessment prior to the commercialization of GE crops. We produced F1 and F2hybrid descendants from crosses of two insect-resistant GE rice lines(Bt, Bt/Cp TI)and their non-GE rice parent with a wild rice(Oryza rufipogon) population to estimate the transgenic fitness. Insect damages and life-cycle fitness of GE and non-GE crop–wild hybrid descendants as well as their wild parent were examined in a common-garden experiment. No significant differences in insect damages were observed between the wild rice parent and GE hybrid descendants under highinsect pressure. The wild parent showed significantly greater relative survival-regeneration ratios than its GE and non-GE hybrid descendants under both high- and low-insect pressure. However, more seeds were produced in GE hybrid descendants than their non-GE counterparts under high-insect pressure. Given that the introduction of Bt and Bt/Cp TI transgenes did not provide greater insect resistance to crop–wild hybrid descendants than their wild parent, we predict that transgene flow from GE insect-resistant rice to wild rice populations may not cause considerable ecological risks.

关 键 词：Environmental impact Fecundity andsurvival Genetically modified organisms Lepidopteran - Oryza sativa Wild relative 

分 类 号：S511[农业科学—作物学]