骨氟化物水平的干燥蒸馏-氟电极测定法的实验条件研究  

Study on experimental condition for modified method of drying and distillation in determining fluoride levels in bone by fluoride ion selective electrode

作　　者：周洲[1] 王红梅[1] 张晗[1] 陈艳卿[1] 

机构地区：[1]环境基准与风险评估国家重点实验室,中国环境科学研究院,北京100012

出　　处：《职业与健康》2016年第17期2356-2359,共4页Occupation and Health

基　　金：中央级公益性科研院所基本科研业务专项支持(2012ZX07101-002)

摘　　要：目的建立一种较为准确、实用的骨样本氟测定预处理方法。方法应用课题研发的新型预处理装置对已110℃干燥4 h后粉碎的骨样本进行酸蒸馏处理,使用拉丁方设计,以回收率作为选择实验条件的指标,对骨样本氟测定的预处理方法进行条件因素（硫酸加入量、淋洗液及接收液碱度值）的实验研究;并应用探索出的最佳实验条件对氟暴露大鼠的骨样本进行准确度和精密度测定。结果当硫酸加入量为200μl、淋洗液与接收液碱度均为1.2 mol/L时,样本组测定的平均回收率最高,为（96.67±6.04）%,即选定该条件为最佳测定实验条件;应用上述最佳实验条件对骨样本进行准确度和精密度检测,方法检测的相对标准偏差（RSD）均在5.5%以内,回收率为94%~102%。结论干燥蒸馏-氟电极法具有较好的准确度和精密度,且与其他传统方法相比,实验装置简单小巧,样本及药剂使用量少,并可实现大批量的实验测定,具有良好的实际应用前景。[Objective] To establish a simple and suitable pre-treatment method for determining the contents of fluoride in bone sample.[Methods]The samples were dried under 110 ℃ for 4 hours and distilled by the new device which developed by our research group before,and then the fluoride contents were determined in 3 parallel tests under different volume of H2SO4,different molarity of leacheate and receptor fluid,adopting Latin square design and rate of recovery as the indexes of the research. The bone samples of rats exposed to fluoride were detected under the optimal experimental conditions,to analyze the accuracy and precision.[Results]The average value of recovery was highest（96.67±6.04）% when the experiment condition was200 μl H2SO4,1.2mol/L Na OH solution of leacheate and receptor fluid,which was regarded as the optimal conditions for determining of fluoride content. The relative standard deviations（RSD）were less than 5.5 % and the range of recovery rate was from 94 % to 102 % when applying the above experiment conditions.[Conclusion]The method describes above is simple and suitable with well accuracy and precision. Compared to the traditional methods,it has a good application prospect for saving the usage amount of the sample and reagent,as well as the realization of the quantity parallel test.

关 键 词：骨 氟化物 干燥蒸馏法 离子选择电极 

分 类 号：R115[医药卫生—公共卫生与预防医学]