周年温度变化对泡桐丛枝病植原体分布和消长的影响  

Impact of Annual Temperature Variation on Distribution and Year-round Concentration Variation of Paulownia Witches' Broom Phytoplasma

作　　者：曹亚兵[1] 翟晓巧[2] 介大委[1] 范国强[1] 

机构地区：[1]河南农业大学泡桐研究所,河南郑州450002 [2]河南省林业科学研究院,河南郑州450008

出　　处：《河南农业科学》2016年第10期85-88,97,共5页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30271082;30571496);高等学校博士学科点专项科研基金项目(20050466003)

摘　　要：为探讨温度变化对泡桐树体内丛枝病植原体分布和消长的影响,利用巢式PCR和直接PCR研究了植原体在泡桐不同器官内的分布及相对含量的周年变化。结果表明,不同发病程度泡桐中丛枝病植原体的分布不同,发病程度相同泡桐不同器官内植原体含量也存在一定差异。在中等发病程度的泡桐中,丛枝病植原体全年存在于枝条内,并且其含量随温度升高逐渐升高,8月份达到最高,此后开始下降;叶片内植原体含量随温度升高明显增加,7月份含量最高,随后减少,10月份降到最低;根部植原体含量随温度升高也相应增加,在9月份达到最高,之后开始降低,全年含量变化较小,且含量最高值较叶片和枝条中低。表明泡桐丛枝病植原体在寄主体内的消长与温度的周年变化关系紧密。To explore the effect of temperature variation on the distribution of witches＇ broom phytoplasma and its annual changes of relative content in paulownia,direct-RCR and nest-PCR were used to research the seasonal variation of the phytoplasma content and distribution. Our result showed that the distribution of phytoplasma in varying degree of diseased paulownia was different,meanwhile the concentration in different organs of the same plant was not the same. In the moderately diseased plant,phytoplasma survived all the year round in branches and increased gradually with the rising of temperature. The highest concentration of phytoplasma appeared in August,and subsequently,the concentration of phytoplasma gradually decreased. The similar situation appeared in leaves,except that the concentration of phytoplasma reached the maximum in July and the minimum in October. In root,the phytoplasma concentration was lower and changed less than in leaves and branches,which reached the maximum in September,and then reduced. In all,the relationship between the variation of phytoplasma concentration in paulownia and annual changes of temperature was very close.

关 键 词：泡桐丛枝病植原体 PCR 温度 消长 

分 类 号：S763.1[农业科学—森林保护学]