基于烟草叶绿体基因组和线粒体基因组SSR标记的烟属植物遗传多样性分析  

Genetic diversity analysis of genus Nicotiana based on SSR markers in chloroplast genome and mitochondria genome

作　　者：曾建敏[1] 陈学军[1] 吴兴富[1] 焦芳婵[1] 肖炳光[1] 李永平[1] 童治军[1] 

机构地区：[1]云南省烟草农业科学研究院,烟草行业烟草生物技术育种重点实验室,国家烟草基因工程研究中心,昆明650021

出　　处：《中国烟草学报》2016年第4期89-97,共9页Acta Tabacaria Sinica

基　　金：中国烟草总公司项目(110201402002、110201302005);中国烟草总公司云南省公司项目(2014YN04、2013YN09)

摘　　要：利用本实验室前期开发的烟草叶绿体基因组和线粒体基因组SSR标记,对属于3个烟草亚属、9个组共35份野生烟草种质资源进行了遗传多样性分析。利用20对SSR引物在35份野生烟草材料中共扩增获得176个多态性条带,每对SSR引物可检测的多态性条带数为2~19个,平均为8.8个。35份材料间遗传相似性系数（GS）在0.20~1.00之间,平均为0.57,表明烟属种间的遗传多样性丰富,遗传差异较大,亲缘关系较远。虽然3个亚属间的界限不明显,但在适当的遗传相似性系数条件下,除香甜烟草组外,35份野生烟草按其组可清晰地聚为7类,表明2种细胞器基因组SSR标记适合用于烟草种间进化、分类、遗传分析等方面研究。Based on SSR markers in chloroplast genome and mitochondrial genome of tobacco, genetic diversity of 35 wild tobacco germplasm belonging to 3 subgenus and 9 sections was first analyzed in this study. 20 primer pairs were selected from 764 tobacco organelle genomic SSR markers which were previously developed by lab. A total of 176 polymorphic bands were amplified in 35 wild Nicotiana species using 20 SSR primers, and the number of polymorphic bands detected by each SSR primer ranged from 2 to 19, with an average of 8.8 polymorphic bands per marker. Genetic similarity coefficient（GS） among the 35 wild Nicotiana species ranged from 0.20 to 1.00, with an average of 0.57, which indicated the genetic variation of the 35 wild Nicotiana species was quite large and the genetic diversity was much abundant in Nicotiana genus. Except for Suaveolentes section, the 35 wild Nicotiana species were clustered into 7 categories by an appropriate genetic similarity coefficient, while the boundary of three subgenus was not obvious. Results indicated that two kinds of tobacco organelle genomic SSR markers in this study are suitable for the evolution, classification, and genetic analysis among different tobacco species.

关 键 词：野生烟草 遗传多样性分析 简单序列重复标记 

分 类 号：S572[农业科学—烟草工业]