脑脊液流式细胞术在儿童中枢神经系统白血病早期诊断中的意义  

Value of Cerebrospinal Fluid Detection with Flow Cytometry in Central Nervous System Leukemia

作　　者：安美玉[1] 陈芬[1] 

机构地区：[1]新疆维吾尔自治区人民医院儿科,新疆乌鲁木齐830000

出　　处：《中外医疗》2016年第29期42-44,共3页China & Foreign Medical Treatment

摘　　要：目的探讨脑脊液流式细胞术(Flow Cytometry,FCM)在诊断儿童中枢神经系统白血病(Central Nervous System Leukemia,CNSL)中的意义。方法采用单克隆抗体双重直接免疫荧光素标记法对2015年1月—2016年3月期间该院收治的45例可疑中枢神经系统浸润患儿的脑脊液进行细胞免疫表型分析,同时进行脑脊液细胞形态学(Conventional Cytology,CC)检测,比较两种方法的差异。结果 45例患儿中,FCM有15例发现异常免疫表型,阳性率为33.33%,CC发现9例存在原始幼稚细胞,阳性率为20%,两者差异具有统计学意义(P<0.01)。结论脑脊液FCM在儿童CNSL的诊断中具有重要的临床意义。Objective To evaluate the value of flow cytometric of cerebrospinal fluid cells in the diagnosis of central nervous system leukemia in children. Methods Used Double immunofluorescence staining with monoclonal antibodies,45 cerebrospinal fluid samples who had central nervous system involvement was analyzed with flow cytometric immunophenotyping and conventional cytology in January 2015 to March 2016,the results of flow cytometry were compared with conventional cytology. Results The results showed that 33.33% children had abnormal cells by flow cytometry and 20% children had abnormal cells by conventional cell morphology in 45 cases。The difference had statistical significance(P < 0.01). Conclusion Flow cytometriy analysis of cerebrospinal fluid has important clinical significance in the diagnosis of CNSL in children。

关 键 词：流式细胞术 细胞形态学 脑脊液 中枢神经系统白血病 

分 类 号：R725.5[医药卫生—儿科]