魁蚶AFLP反应体系的建立  

The establishment of AFLP reaction system for Scapharca broughtonii

作　　者：姚红伟[1] 高悦勉[1] 

机构地区：[1]大连海洋大学,辽宁大连116023

出　　处：《河北渔业》2016年第10期1-4,共4页

基　　金：科技部支撑计划(2006BAD09A15)资助

摘　　要：以魁蚶为研究材料,建立了魁蚶的AFLP分子标记反应体系。对其分析过程中的酶切链接、预扩增、选择性扩增等试验条件进行了检测与筛选,得到了清晰的AFLP指纹图谱,为探讨魁蚶种群之间的遗传关系和遗传图谱的构建等方面提供了新的技术手段。The AFLP reaction system of Scapharca broughtonii was established. The experiment conditions about double digestion and ligation, pre -amplification, selective amplification were tested and filtered. A clear DNA fingerprints was obtained. This provided a new technique method for probing the genetic relationship among populations of Scapharca broughtonii and constructing their genetic maps.

关 键 词：魁蚶 AFLP 反应体系 

分 类 号：S917.4[农业科学—水产科学]