裸花紫珠联合万古霉素对耐甲氧西林金黄色葡萄球菌致大鼠肺炎模型的协同抗菌作用研究  

Synergistic antibacterial effect of association of Callicarpa nudiflora and Vancomycin Hydrochloride towards rat model of MRSA pneumonia

作　　者：谢泳超 谷陟欣 朱丽[1] 黄胜 钟志红[1] 宁宁[1] 冯星[1] 

机构地区：[1]湖南师范大学医学院药学系,湖南长沙410013 [2]九芝堂股份有限公司,湖南长沙410000

出　　处：《中草药》2016年第17期3070-3073,共4页Chinese Traditional and Herbal Drugs

基　　金：湖南省中医药科研计划项目重点项目(201204);湖南省科技厅项目(2012RS4020;2014SK3238);长沙市科技局一般项目(K1203004-31);海南省中药现代化专项(2012ZY020)

摘　　要：目的观察裸花紫珠联合万古霉素对耐甲氧西林金黄色葡萄球菌(MRSA)所致大鼠肺炎模型的协同抗菌作用,为临床上治疗MRSA所致的感染提供一种更安全有效的治疗方法。方法将80只健康雄性SD大鼠随机分成8组,10只作为对照组,其余大鼠从左侧鼻腔分别滴入200μL MRSA(1×108 CFU/m L)菌液造模。每天记录观察饮食、饮水量情况及大鼠活动度,持续给药10 d后观察大鼠血液白细胞数、肺部载菌量和组织病理学变化。结果裸花紫珠联合盐酸万古霉素各组与模型组相比,白细胞计数均下降明显(P<0.05);大鼠肺部的载菌量明显降低(P<0.05),肺组织病理损伤程度均明显减轻。结论裸花紫珠联合盐酸万古霉素对MRSA有协同抗菌作用,能更好地治疗MRSA引起的肺炎,可提高抗生素对耐药菌疗效,缩短疗程。Objective To investigate the synergistic antibacterial effect of Callicarpa nudiflora associated with Vancomycin Hydrochloride on the pneumonia model rats infected by methicillin-resistant staphylococcus aureus（MRSA）, and to provide a safer and more effective treatment of clinical ideas for the treatment of infections caused by MRSA. Methods Totally 80 NIH female rats were randomly divided into eight groups, ten rats as the control group, and 200 μL MRSA（1 × 108 CFU/ m L） bacteria was dropped in the left nasal cavity of the remaining rats to produce infected animal model. The administration with drug respectively by group was continued for 10 d. The common state including activity and intake of water and food were observed and noted. After administration for 10 d, the count of leucocyte, microbial load, and histopathological change of lung were observed. Results Compared with the model group, the absolute value of leucocyte and microbial load of association of C. nudiflora and Vancomycin Hydrochloride were reduced signicantly（P 〈 0.05）. The pathological damage alleviated significantly. Conclusion Association of C. nudiflora and Vancomycin Hydrochloride has the synergistic antibacterial effect towards MRSA, which can improve the curative effect of antibiotics and shorten the period of treatment.

关 键 词：裸花紫珠 盐酸万古霉素 耐甲氧西林金黄色葡萄球菌 协同抗菌作用 肺炎 

分 类 号：R285.5[医药卫生—中药学]