一株油茶炭疽病病原拮抗细菌的分离与鉴定  

Isolation and identification of an antagonistic bacteria to Colletotrichum gloeosporioides of Camellia oleifera

作　　者：吴天乐[1] 陈艺[1] 马力[1] 曾粮斌[2] 李良导[3] WU Tianle CHEN Yi MA Li ZENG Liangbin LI Liangdao(Hunan Academy of Forestry, Changsha 410004, China Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China Hunan Women＇s University, Changsha 410004, China)

机构地区：[1]湖南省林业科学院,湖南长沙410004 [2]中国农业科学院麻类研究所,湖南长沙410205 [3]湖南女子学院,湖南长沙410004

出　　处：《湖南林业科技》2016年第5期62-66,共5页Hunan Forestry Science & Technology

基　　金：基金项目:国家林木种质资源平台建设(2016DKA210003)

摘　　要：采用梯度稀释涂板法和平板对峙培养法从油茶林根际土中分离、筛选到了一株对油茶炭疽病原具有拮抗作用的细菌,编号为YCSF-1,其对油茶炭疽病病原的抑菌圈直径达20.6 mm。经过形态学、生理生化测定、16Sr RNA序列比对鉴定,该菌株为侧孢短芽孢杆菌(Brevibacillus laterosporus)。该研究为油茶炭疽的生物防治提供菌株。A strain of bacteria was isolated from the rhizosphere soil of Camellia oleifera using plate dilution method and plate confrontation culture method, which had antagonistic effect on Colletotrichum gloeosporioides of Camellia oleifera. The diameter of the inhibition zone was 20. 6mm. After morphological and 16 Sr RNA sequence alignment,this strain was identified as Brevibacillus laterosporus. The research provided the strains for the biological control of Colletotrichum gloeosporioides of Camellia oleifera.

关 键 词：油茶 炭疽病 拮抗细菌 鉴定 

分 类 号：S763.7[农业科学—森林保护学]