水稻新生多肽结合复合体α链基因家族的表达分析  

Expressional Analysis of Osα-NAC in Rice

作　　者：王晓静[1] 孙林静[1] 马忠友[1] 孙玥[1] 李军玲[1] 张融雪[1] 闫双勇 孙卫宁[2] WANG Xiaojing SUN Linjing MA Zhongyou SUN Yue LI Junling ZHANG Rongxue YAN Shuangyong SUN Weining(Tianjin Institute of Crop,Tianjin 300384,China Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China)

机构地区：[1]天津市农作物研究所,天津300384 [2]中国科学院上海生命科学研究院植物生理生态研究所,上海200032

出　　处：《华北农学报》2016年第4期1-6,共6页Acta Agriculturae Boreali-Sinica

基　　金：天津市农业科学院院长基金项目(15005);国家科技支撑计划项目(2013BD0508);农业部转基因专项(2011ZX08001-004)

摘　　要：水稻新生多肽结合复合体α链(Osα-NAC)基因家族共有3个成员,分别位于第1,3,5号染色体。为研究Osα-NAC基因家族在逆境环境中的生物学功能,利用RT-q PCR和Western Blot技术,详细分析了Osα-NAC基因家族在不同组织中的表达情况,并进一步研究了Osα-NAC基因家族对非生物胁迫的应答模式。结果表明,在高盐和模拟干旱条件下,Os1g NAC和Os5g NAC基因表达上调,Os3g NAC基因表达下调,提示Osα-NAC基因家族各成员在水稻响应非生物胁迫的过程中发挥不同的作用。上述试验结果初步阐明该基因家族在非生物胁迫下的表达特性,为今后更深入研究该基因家族的功能提供了理论依据。The Osα-NAC（ nascent polypeptide-associated complex alpha chain） gene family contains three genes which are localized on chromosome 1,3,and 5,respectively. To analyse the function of Osα-NAC under environmental stresses,the expression patterns of Osα-NAC to various abiotic stress were analyzed. After salt and drought treatment,Os1 g NAC and Os5 g NAC were up-regulated while Os3 g NAC was down-regulated,suggesting that different members of Os-NAC gene family have different function in stress response. The results of this study revealed the expression patterns of Osα-NAC gene family under abiotic stresses,and provided some clues to the function of Osα-NAC for the further research.

关 键 词：α-NAC 基因表达 蛋白表达 逆境胁迫 

分 类 号：S511.03[农业科学—作物学] Q78[生物学—分子生物学]