中药复方护骨胶囊对去卵巢大鼠骨骼结构和骨重建状态的影响  

Effects of herbal compound Hugu capsules on bone structure and bone remodeling in ovariectomized rats

作　　者：林泽苗 钟佳贤 贾欢欢[2] 吴玉娥[2] 陈珺[1] 李青南[1,2] 

机构地区：[1]广东药科大学生命科学与生物制药学院,广州510006 [2]广东省实验动物重点实验室广东省实验动物监测所,广州510663

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2016年第3期296-303,共8页Chinese Journal Of Osteoporosis And Bone Mineral Research

基　　金：广东省科技计划国际合作项目(2010B050700022)

摘　　要：目的观察护骨胶囊(Hugu capsules,HG)对去卵巢大鼠骨结构和骨重建状态的影响。方法 3月龄SPF级雌性SD大鼠60只采用数字表法随机分为假手术组,去卵巢组,仙灵骨葆胶囊组和护骨胶囊低、中、高剂量组(Sham、OVX、Pos、HG-L、HG-M和HG-H)。大鼠切除卵巢后,隔天开始给药,连续灌胃3个月,处死前进行两次荧光标记。取大鼠胫骨上段(proximal tibia,PTM)与中段(tibia shaft,TX)进行骨形态计量学分析。结果与Sham组比较:OVX组松质骨的骨小梁面积百分率(percent trabecular bone area,Tb.Ar)和骨小梁数量(trabecular number,Tb.N)分别降低66.68%和65.01%,骨小梁分离度(trabecular seperation,Tb.Sp)增加276.97%。单位骨小梁周长成骨细胞数(osteoblast number/unit trabecular perimeter,Ob.N)和成骨细胞周长百分率(percent osteoblast perimeter,Ob.Pm)分别增加357.53%和468.27%,单位骨小梁周长破骨细胞数(osteoclast number/unit trabecular perimeter,Oc.N)和破骨细胞周长百分率(percent osteoclast perimeter,Oc.Pm)分别增加213.95%和216.67%,骨形成率-体积(bone formation rate/volume,BFR/BV)增加39.12%,骨形成率-组织(bone formation rate/tissue,BFR/TV)降低55.56%,提示松质骨骨质疏松造模成功。皮质骨没有变化;与OVX组比:HG组大鼠松质骨的Tb.Ar、Tb.N和骨小梁宽度(trabecular width,Tb.Wi)增加,Tb.Sp、Oc.N、Oc.Pm降低,荧光周长百分率(percent fluorescence perimeter,L.Pm)、骨形成率-周长(bone formation rate/bone surface,BFR/BS)、BFR/BV和骨形成率-组织(bone formation rate/tissue,BFR/TV)增加。HG组大鼠皮质骨的T.Ar、Ct.Ar、骨外膜荧光标记周长百分率(percent periosteal fluorescence perimeter,P-L.Pm)和骨外膜骨形成率(periosteal bone formation rate/bone surface,P-BFR/BS)均增加。说明HG能增加松质骨骨量,改善骨结构,促进骨形成,抑制骨吸收,且增加皮质骨骨量,促进皮质骨骨外膜骨形成;与Pos组相比:HG-H组的松质骨Tb.N增加23.80%,Tb.Sp降低22.71%,HG-L和HG-M组�Objective To observe the effects of Hugu capsules（ HG） on bone structure and bone remodeling in ovariectomized rats. Methods Sixty 3-month-old female SD rats（ SPF） were distributed randomly into 6 groups： Sham group,OVX group,Xianlinggubao group（ positive control） and HG low,medium and high dose group（ Sham,OVX,Pos,HG-L,HG-M,HG-H）. After ovariectomy,the next day,the rats were ig administrated medicine continuously for three months and fluorescently labeled twice before being killed. Rat proximal tibia（ PTM） and tibia shaft（ TX） were took for bone histomorphometry study. Results Compared with Sham group： At PTM,the percent trabecular bone area（ Tb. Ar） and trabecular number（ Tb. N） of OVX group decreased by 66. 68% and 65. 01% respectively,and trabecular seperation（ Tb. Sp） increased by 276. 97%. Osteoclast number / unit trabecular perimeter（ Ob. N） and percent osteoblast perimeter（ Ob. Pm） increased by 357. 53% and 468. 27% respectively,osteoclast number / unit trabecular perimeter（ Oc. N） and percent osteoclast perimeter（ Oc. Pm） increased by 213. 95% and 216. 67% respectively,bone formation rate / volume（ BFR / BV） increased by 39. 12%,bone formation rate / tissue（ BFR / TV） decreased by 55. 56%,which suggested that the cancellous bone and osteoporosis model was successful. TX had no change; Compared with OVX group： At PTM,the Tb. Ar,Tb. N and Tb. Wi of HG groups were increased,the Tb. Sp,osteoclast number / unit trabecular perimeter（ Oc. N） and percent osteoclast perimeter（ Oc. Pm） were reduced,percent fluorescence perimeter（ L. Pm）,bone formation rate/bone surface（ BFR/BS）,BFR/BV and BFR/TV were increased. At TX,the T. Ar,Ct. Ar,percent periosteal fluorescence perimeter（ P-L. Pm） and periosteal bone formation rate / bone surface（ P-BFR /BS） of HG groups were increased. It showed that HG can increase the PTM bone mass,improve the bone structure,promote bone formation,inhibit bone resorption,and increased the TX bone mass,

关 键 词：护骨胶囊 骨质疏松 松质骨 皮质骨 

分 类 号：R681[医药卫生—骨科学]