阜阳市120例发热伴呼吸道症候群患者细菌学诊断与结果分析  

Bacteriologic diagnosis and results analysis of 120 patients with febrile respiratory syndrome in Fuyang

作　　者：卜戈[1] 李国兰[1] 赵蕾[1] 孟昭倩[1] 朱义朗[2] 杨理理[2] 

机构地区：[1]阜阳市疾病预防控制中心,安徽阜阳236000 [2]阜阳市第二人民医院,安徽阜阳236000

出　　处：《中国卫生检验杂志》2016年第17期2480-2481,2486,共3页Chinese Journal of Health Laboratory Technology

基　　金：基金项目:安徽省细菌性传染病病原谱流行规律与变异研究(20-13ZX10004-203-002)

摘　　要：目的明确阜阳市发热伴呼吸道症候群患者细菌学病原谱。方法检测120例发热伴呼吸道患者标本,用荧光定量PCR方法,检测金黄色葡萄球菌、肺炎链球菌、肺炎克雷伯菌、流感嗜血杆菌、铜绿假单胞菌、乙型溶血性链球菌、军团菌7种病原;咽拭子标本用荧光定量PCR方法,检测肺炎支原体、肺炎衣原体病原;尿液标本用免疫胶体金方法,检测肺炎链球菌、军团菌病原。结果 120份血液标本培养全为阴性;120份痰液标本培养出金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌各1株,培养阴性痰标本做核酸检测后测序,最终阳性以3例培养阳性和测序阳性为准;97份咽拭子标本,以PCR结果为最终阳性判断依据,肺炎支原体阳性率为2.06%,肺炎衣原体阴性;120份尿液标本,肺炎链球菌抗原阳性率为8.33%,军团菌为阴性。患者病原检出阳性率为24.17%。结论阜阳市发热伴呼吸道症候群患者致病菌感染主要以肺炎链球菌、铜绿假单胞菌、流感嗜血杆菌、肺炎支原体为主。Objective To identify the etiological spectrum in patients with febrile respiratory syndrome in Fuyang. Methods Specimens from 120 cases with febrile respiratory syndrome were detected,and 7 kinds of pathogens including Staphylococcus aureus,Streptococcus pneumoniae,Klebsiella pneumoniae,Haemophilus influenzae,Pseudomonas aeruginosa,β-Hemolytic streptococcus,Legionella were detected by fluorescence quantitative PCR. Pharyngeal swab specimens were detected using fluorescent quantitative PCR for Mycoplasma pneumoniae and Chlamydia pneumoniae; urine samples were detected using immune colloidal gold method for Streptococcus pneumoniae and Legionella. Results 120 blood samples were all negative; Staphylococcus aureus,Pseudomonas aeruginosa,Klebsiella pneumoniae were cultivated each 1 strain from 120 sputum specimens.Negative sputum specimens were cultured for nucleic acid detection and then for sequencing. The final positive were subjected by 3 culture positive and 3 sequencing positive; as for the 97 throat swab specimens,the final positive was based on the positive PCR results; the positive rate of Mycoplasma pneumoniae was 2. 06%,Chlamydia pneumoniae was negative; as for the 120 urine specimens,the positive rate of Streptococcus pneumoniae antigen was 8. 33%,and Legionella bacteria were negative. The positive rate of pathogen detection in patients were 24. 17%. Conclusion The main pathogenic bacteria in febrile respiratory syndrome were Streptococcus pneumoniae,Pseudomonas aeruginosa,Haemophilus influenzae,Mycoplasma pneumoniae in Fuyang.

关 键 词：发热伴呼吸道症候群 细菌 诊断 

分 类 号：R446.5[医药卫生—诊断学]