血清二氧化碳逸散对检测结果的影响分析  

Analysis of the influence of carbon dioxide loss in serum on the test results

作　　者：闫景刚[1] 许新春[1] 李豹[1] 陈峰[1] 

机构地区：[1]内蒙古医学院第四附属医院检验科,内蒙古包头014030

出　　处：《中国卫生检验杂志》2016年第17期2512-2513,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨不同环境温度血清放置不同时间,血清中二氧化碳逸散对检测结果影响。方法促凝剂试管采集静脉血液,离心机分离血清,酶法测定,用同质血清在19℃～20℃条件下,分1 min、120 min、240 min 3次进行重复检测;在27℃～28℃条件下,分1 min、30 min、60 min 3次检测,分别观察血清中二氧化碳逸散。结果在19℃～20℃条件下120 min内及27℃～28℃条件下30 min内,二氧化碳逸散差异无统计学意义（P〉0.05）;而在19℃～20℃条件下放置240 min及在27℃～28℃条件下放置60 min,检测结果差异有统计学意义（P〈0.01）。结论血清中二氧化碳检测结果,受环境温度及放置时间等因素影响,环境温度低时血清二氧化碳逸出较慢,温度升高血清二氧化碳逸出加快,因此在日常工作中,需根据环境温度及样本待检时间,进行相关降温处理,防止检测结果降低影响临床诊断。Objective To discuss how does the loss of CO2 influence the test results in blood serum under different temperature. Methods Collect the venous blood with promote agglutination test tube,separate the serum with centrifuge,and use enzymatic determinsation. Repeat the tests by 1 min,120 min,240 min during 19 ℃-20 ℃ with the homogeneous serum,test by 1 min,30 min,60 min during 27 ℃-28 ℃,and then observe the loss of CO2. Results Under the temperature of 19 ℃-20 ℃ for 120 min,or the temperature of 27 ℃-28 ℃ for 30 min,there was no significant change on the loss of CO2（ P〉0. 05）; while under the temperature of 19 ℃-20 ℃ for 240 min and of 27 ℃-28 ℃ for 60 min,the difference of test results had statistical significance（ P〈0. 01）. Conclusion The test results of CO2 in the serum are influenced by temperature and time. The lower temperature could slow the loss of CO2. Raising the temperature could accelerate the loss of CO2. In daily work,necessity cooling measure should be taken according to temperature and time,so as to avoid the decrease of test results influencing the diagnosis.

关 键 词：总二氧化碳 环境温度与时间 结果影响 

分 类 号：R446.1[医药卫生—诊断学]