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作 者:王雪晶[1,2,3] 罗鑫[1,2,3] 周建明[1,2,3] 宋亚玲[1,2,3] 赵祎武[1,2,3] 黄文哲[1,2,3] 王振中[1,2,3] 萧伟[1,2,3]
机构地区:[1]江苏康缘药业股份有限公司,江苏连云港222000 [2]中药制药过程新技术国家重点实验室,江苏连云港222000 [3]中药提取精制新技术重点研究室,江苏连云港222000
出 处:《中草药》2016年第16期2822-2826,共5页Chinese Traditional and Herbal Drugs
基 金:科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)
摘 要:目的对大株红景天Rhodiola wallichiana var.cholaensis根茎的化学成分进行研究,并探讨其对心肌细胞H9c2损伤的保护作用。方法采用动态轴向压缩柱色谱、硅胶柱色谱以及HPLC等方法进行分离纯化,利用现代波谱学技术鉴定化合物结构。对分离得到的化合物进行细胞损伤保护活性实验。结果从大株红景天根茎中共分离并鉴定了16个单体化合物,分别为红景天苷(1)、没食子酸(2)、没食子酸甲酯(3)、槲皮素(4)、邻苯三酚(5)、6″-O-galloylsalidroside(6)、没食子酸乙酯(7)、山柰酚-7-O-α-L-鼠李糖苷(8)、草质素-7-O-β-D-吡喃葡萄糖苷(9)、草质素-7-O-(3″-O-β-D-葡萄糖基)-α-L-鼠李糖苷(10)、山柰酚-3-O-β-D-吡喃葡萄糖苷(11)、草质素(12)、草质素-7-O-α-L-鼠李糖苷(13)、小麦黄素(14)、芦丁(15)、山柰酚-3-O-(2″-O-β-D-木糖基)-β-D-葡萄糖苷(16)。样品质量浓度为25μg/m L时,化合物2、6、8、12和15对心肌细胞H9c2的保护率分别为20.40%、31.54%、67.61%、44.27%和47.84%。结论化合物5为首次从红景天属植物中分离得到,化合物7~16为首次从该植物中分离得到。化合物2、6、8、12和15在一定程度上对心肌细胞H9c2损伤具有保护作用。Objective To investigate the chemical constituents from the rhizomes of Rhodiola wallichiana var. cholaensis and their protective effects on myocardium of H9c2 cells. Methods The compounds were isolated by dynamic axial compression chromatography, silica gel column chromatography, and HPLC. Their structures were identified on the basis of spectral data analysis. The protective effects of aU isolated compounds on myocardium were determined. Results Sixteen compounds were isolated from the rhizomes ofR. walliehiana var. cholaensis and identified as salidroside (1), gallic acid (2), methyl gallate (3), quercetin (4), pyrogallol (5), 6"-O-galloylsalidroside (6), ethyl gallate (7), kaemnpferol-7-O-α-L-rhamnopyranoside (8), herbacertin-7-O-β-D-glucopyranoside (9), rhodiosin (10), kaemnpferol-3-O-β-D-glucopyranoside (11), herbacetin (12), herbacertin-7-α-L-rhamnopyranoside (13), tricin (14), rutin (15), and kaemnpferol-3-O-(2"-O-β-D-xylosyl)-β-D-glucopyranoside (16). Compounds 2, 6, 8, 12, and 15 showed the significant protective effects against H9c2 cells at the concentration of 25 μg/mL and the protective ratios were 20.40%, 31.54%, 67.61%, 44.27%, and 47.84%. Conclusion Compound 5 is isolated from the species of genus Rhodiola L. for the first time, and compounds 7--16 are obtained from the plant for the first time. Compounds 2, 6, 8, 12, and 15 could protect the myocardium of H9c2 cells to some extent.
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