服用温度对葛根芩连汤治疗大鼠溃疡性结肠炎药效的影响  

Effect of administration temperature on efficacy of Gegen Qinlian Decoction on DSS-induced ulcerative colitis in rats

作　　者：高观祯[1] 孔玉婷 汪惠勤[1] 禄彦科 周建武[1] 柯李晶[1] 饶平凡[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310035

出　　处：《中草药》2016年第16期2897-2901,共5页Chinese Traditional and Herbal Drugs

基　　金：浙江省食品科学与工程"重中之重"一级学科开放研究基金(JYTsp20141072);浙江工商大学引进人才科研启动费(引13-15;引13-17);国家重点基础研究发展计划"973"项目(2010CB530605)

摘　　要：目的考察服用温度是否影响葛根芩连汤对溃疡性结肠炎模型大鼠的药效。方法以葡聚糖硫酸钠(DSS)诱导溃疡性结肠炎(UC)大鼠模型,不同温度(10、42℃)的葛根芩连汤ig给药,连续给药7 d,进行疾病活动指数(DAI)评分,比较给药前后结肠大体形态和病理学改变,比较各组大鼠血清及结肠组织中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷光甘肽过氧化物酶(GHS-Px)、肿瘤坏死因子-α(TNF-α)等生化指标的变化。结果与对照组比较,模型组大鼠出现明显的UC相关症状及指标改变,葛根芩连汤低温组(10℃)及高温组(42℃)均显示出明显的治疗效果;其中,葛根芩连汤低温组在病理组织学评分、肠壁厚度和多项生化指标上均明显好于高温组,显示出更好的治疗效果。结论针对UC大鼠模型,葛根芩连汤冷服的效果优于热服,推测可能与不同温度诱发肠道黏膜肥大细胞不同强度的应激反应有关。Objective To observe the effect of the administration temperature of GQD on the efficacy to ulcerative colitis （UC） of rats. Methods The GQD was given to dextran sulfate sodium （DSS）-induced UC rats at different temperature （10 ℃/42℃） for 7 d. The disease activity index （DAI）, histopathological score of colon, superoxide dismutase （SOD）, malondialdehyde （MDA）, GHS-Px, myeloperoxidase （MPO） of serum and colon tissue, and the tumor necrosis factor （TNF-ct） of serum were measured and observed. Results Compared with the control group, the UC relative symptom and index changes of rats in the model group appeared obviously. GQD showed the significant therapeutic effects in both 10 ℃ and 42℃groups. The lower temperature administration achieved significantly better results than the higher one on the histopathological score, colon wall thickness, and biochemical indices. Conclusion The cold GQD shows higher efficacy than the hot GQD when treating UC, which may be attributed to the immune response of intestinal mucosal mast cells to temperature-induced stress.

关 键 词：葛根芩连汤 服用温度 葡聚糖硫酸钠 溃疡性结肠炎 疾病活动指数评分 髓过氧化物酶 超氧化物歧化酶 丙二醛 谷光甘肽过氧化物酶 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]