甘油基混合培养物合成PHA及其与OUR的关系  

Polyhydroxyalkanoate( PHA) Synthesis by Glycerol-based Mixed Culture and Its Relation with Oxygen Uptake Rate( OUR)

作　　者：刘东[1] 张小婷[1] 张代钧[1,2] 曾善文[1] 卢培利[1,2] 

机构地区：[1]重庆大学环境科学系,重庆400044 [2]煤矿灾害动力学与控制国家重点实验室,重庆400044

出　　处：《环境科学》2016年第9期3518-3523,共6页Environmental Science

基　　金：重庆市自然科学基金项目(CSTC2013JJB20002);煤矿与灾害动力学与控制国家重点实验室科研基金项目(2011DA105287-ZD201505);国家水体污染控制与治理科技重大专项(2012ZX07307-001)

摘　　要：聚β羟基烷酸盐(PHA)以其较好的生物相容性、可生物降解性和再生性成为最有前途的生物高分子材料.本文基于甘油作为基质在饱食-饥饿模式下富集合成PHA的混培物,以乙酸、丙酸、丁酸、乳酸和葡萄糖作为基质考察混培物合成PHA的基质广谱性,结果表明乳酸和乙酸作为基质时PHA的产率系数较高;使用不同比例乙酸/丙酸混合基质时,PHA产量随着乙酸含量的增加而增大,乙酸/丙酸为3∶1时PHA产量最高.通过活性污泥同时贮存与生长模型模拟与线性回归两种方法证实,在单一基质或乙酸/丙酸混合基质情况下,PHA合成速率与OUR存在线性关系,因此,基于在线OUR测量数据可以实时估计PHA合成量.Polyhydroxyalkanoate（ PHA） is one of the most promising biopolymers with the advantages of biodegradable biocompatible and renewable. A glycerol-fed PHA-producing mixed culture was enriched in a feast-famine regime. The substrate versatility and PHA production capacity of the mixed culture were studied using acetate,propionate,butyric,glucose and lactate as substrate,respectively.The results showed a high PHA content with lactate and acetate as the sole substrate,respectively. When acetate and propionate were used as mixed substrates to produce PHA,the PHA contents increased with the proportion of acetate and the PHA content was the highest as the ratio acetate / propionate was 3∶ 1. The results based on the modeling of simultaneously storage and growth and the linear fitting under three different acetate / propionate ratios showed that there was a linear relation between PHA synthesis and OUR. Thus,it is feasible to estimate PHA content based on in-situ real-time monitoring data of OUR.

关 键 词：混合培养物 混合基质 聚β羟基烷酸盐(PHA) 实时在线测量 氧利用速率(OUR) 

分 类 号：X703.1[环境科学与工程—环境工程]