锯叶棕提取物对胶质瘤大鼠免疫的调节  

Regulation of saw palmetto extract on immune function in GL261 glioma

作　　者：沈京莲[1] 张红岩[1] 车玉琴[1] 朱杰[1] 彭沈君[2] 

机构地区：[1]中国医科大学附属第四医院,沈阳110032 [2]辽宁中医药大学,沈阳110032

出　　处：《中国免疫学杂志》2016年第9期1299-1302,共4页Chinese Journal of Immunology

基　　金：辽宁省自然科学基金(2013021078)

摘　　要：目的:观察锯叶棕提取物对大鼠体内胶质瘤的作用及其对免疫系统的影响。方法:制作SD大鼠皮下胶质瘤模型,加空白对照组共为4组,即空白对照组(n=10)、荷瘤组(n=10)、低剂量SR治疗组(n=10)、高剂量SR治疗组(n=10)。大鼠造模后饲养1周开始给予锯叶棕提取物及安慰剂,低剂量组50 mg/kg、隔日灌胃一次,高剂量组300 mg/kg、隔日灌胃1次,其他组给予相同剂量的蒸馏水灌胃。喂养大鼠4周后,处死大鼠后测瘤重、计算抑瘤率,TUNEL染色法检测肿瘤细胞凋亡,巨噬细胞吞噬鸡红细胞活性检测方法检测脾巨噬细胞活性,流式细胞仪检测CD4阳性淋巴细胞。结果:1用药组与荷瘤组比较,瘤重明显减少(F=62.678,P=0.000);高剂量组与低剂量组比较,抑瘤率明显提高。2荷瘤组肿瘤细胞凋亡明显偏少,用药组肿瘤细胞凋亡明显增加,且SR高剂量组细胞凋亡最多(F=1.287,P=0.000)。3荷瘤组与空白组比较,脾巨噬细胞活性明显下降,用药后脾巨噬细胞活性提高,与剂量呈正相关(F=141.205,P=0.000;F=126.903,P=0.000)。4荷瘤组与空白组比较,CD4阳性淋巴细胞百分率减少,用药后CD4阳性淋巴细胞百分率增加,与剂量呈正相关(F=207.294,P=0.000)。结论:锯叶棕提取物能调节机体免疫功能,从而杀伤肿瘤细胞、诱导肿瘤细胞凋亡,药物浓度越大、其作用越明显。Objective：To investigate the effect of saw palmetto extract（SR） on GL261 glioma in rats and immune system.Methods：The 40 rats were divided into 4 groups randomly,one was the control group without tumor（the blank group）（n =10）,the other 30 rats were given subcutaneous inoculation of tumor cells and then divided into 3 groups：the tumor-bearing group（n =10）,low dose SR group（n = 10）,and high dose SR group（n = 10）.After 1 week fed,the rats of SR groups were given the saw palmetto extract,low dose group 50 mg/kg once a day every other day and 300 mg/kg of high dose group every other day.The tumor-bearing groups received the same dose of distilled water.After 4 weeks fed,we measured the tumor weight and the inhibition rate was calculated.The tumor cell apoptosis was detected by TUNEL staining.The activity of splenic macrophages was detected by macrophage phagocytosis of chicken erythrocytes and CD4~＋lymphocytes by flow cytometry.Results：1 The SR groups compared with tumor group,the tumor weight was significantly reduced（F = 62.678,P = 0.000）.The tumor inhibition rate was significantly higher in high dose group.2The apoptosis of tumor cells in tumor-bearing group was significantly less than SR groups and the apoptosis was significantly increased after treatment with SR,especially in high dose SR group（F = 1.287,P = 0.000）.3Compared with the blank group,the macrophage activity decreased remarkablely.SR enhanced the macrophage activity obviously,positively related to doses of SR（F =141.205,P = 0.000;F = 126.903,P = 0.000）.4In the tumor-bearing group,the CD4^＋lymphocyte count decreased significantly.After SR treatment,the CD4^＋lymphocyte increased and were positively related to the dose of SR.Conclusion：Saw palmetto extract can strengthen the immune system.

关 键 词：胶质瘤 锯叶棕提取物(SR) 凋亡 巨噬细胞活性 CD4阳性淋巴细胞 

分 类 号：R739[医药卫生—肿瘤]