戴氏绿僵菌多糖对糖尿病模型小鼠血糖和免疫功能的影响  

Effect of Metarhiziun taii polysaccharide on serum glucose and immune function of diabetic mellitus model mics

作　　者：肖代敏[1] 吕纯莉[1] 肖建辉[2] 曹喻[1] 张志敏[3] 刘民[4] 

机构地区：[1]遵义医学院附属医院医学检验科,遵义563099 [2]遵义医学院附属医院转化医学中心,遵义563000 [3]遵义医学院医学遗传学教研室,遵义563000 [4]遵义医学院检验系,遵义563000

出　　处：《中国免疫学杂志》2016年第9期1338-1341,共4页Chinese Journal of Immunology

基　　金：贵州省科技厅基金(黔科合J字[2009]2148号);贵州省科技厅联合基金(黔科合J字LKZ[2011]35号)项目

摘　　要：目的:探讨戴氏绿僵菌多糖对糖尿病模型小鼠血糖和免疫功能的影响。方法:采用连续多次小剂量腹腔注射链脲佐菌素(STZ)建立Ⅰ型糖尿病模型,观察模型小鼠体重变化,检测空腹血清葡萄糖,免疫器官(脾脏、胸腺)指数,腹腔巨噬细胞吞噬能力,脾淋巴细胞增殖及血清中免疫球蛋白Ig G、补体C3、C4的含量等。结果:戴氏绿僵菌多糖能够改善模型小鼠体重;降低模型小鼠血糖含量,中、高剂量的多糖均能显著提高模型小鼠T、B淋巴细胞的增殖能力(P<0.01);高剂量能显著提高模型小鼠脾脏指数和血清中Ig G、C3、C4的含量(P<0.01);中、高剂量能显著提高模型小鼠胸腺指数及腹腔巨噬细胞吞噬能力(P<0.01)。结论:戴氏绿僵菌多糖可增强STZ诱导的Ⅰ型糖尿病模型小鼠的免疫功能和降低Ⅰ型糖尿病模型小鼠血糖含量。Objective：To investigate the effects of Metarhiziun taii polysaccharide on the immunity activity of the diabetic mellitus model mouse.Methods：To build stables type Ⅰ diabetic model mouse by intraperitoneal injecting small dose streptozotocin（STZ） continuously.Then observeing weight changes,detecting empty stomach serum glucose,immune organ index（include spleen and thymus）,peritoneal macrophage phagocyte ability,spleen lymphocyte multiplication and immunoglobulin Ig G＇s and complement C3,C4 content in blood serum of the diabetic rats.Results：The weight of model mouse had been improved by Metarhiziun taii polysaccharide,serum glucose had been improved degrade of the ability of model mouse.T,B lymphocyte proliferation had been increased obviously by middle and high dose polysaccharid,spleen index,Ig G＇s and C3,C4 content in blood serum of the diabetic rats could be improved by high dose distinctively.Thymus index and the capacity of peritoneal macrophage phagocyte would enhanced by middle and high dose polysaccharide inject.Conclusion：The immune function and serum glucose of type Ⅰ diabetic mode rats could be improved by intragastriced Metarhiziun taii polysaccharide.

关 键 词：戴氏绿僵菌 多糖 糖尿病模型 链脲佐菌素 

分 类 号：R285.5[医药卫生—中药学]